Superresolution by image scanning microscopy using pixel reassignment

被引：180
|
作者：
Sheppard, Colin J. R. [1 ]
Mehta, Shalin B. [2 ]
Heintzmann, Rainer [3 ,4 ,5 ]
机构：
[1] Ist Italiano Tecnol, I-16163 Genoa, Italy
[2] Marine Biol Lab, Woods Hole, MA 02543 USA
[3] Inst Photon Technol, D-07745 Jena, Germany
[4] Univ Jena, Abbe Ctr Photon, Inst Phys Chem, D-07743 Jena, Germany
[5] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England
来源：
OPTICS LETTERS | 2013年 / 38卷 / 15期
关键词：
STRUCTURED ILLUMINATION MICROSCOPY; CONFOCAL MICROSCOPY; SUPER-RESOLUTION;
D O I：
10.1364/OL.38.002889
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84. (C) 2013 Optical Society of America
引用
收藏
页码：2889 / 2892
页数：4
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