Triple-Stranded DNA Containing 8-Oxo-7,8-dihydro-2′-deoxyguanosine: Implication in the Design of Selective Aptamer Sensors for 8-Oxo-7,8-dihydroguanine

被引：35
|
作者：
Zhang, Qian [1 ]
Wang, Yiqi [1 ]
Meng, Xianyang [1 ]
Dhar, Rik [2 ]
Huang, Haidong [1 ]
机构：
[1] New Jersey Inst Technol, Dept Chem & Environm Sci, Newark, NJ 07102 USA
[2] Heritage Inst Technol, Kolkata 700107, India
来源：
ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 01期
关键词：
HPLC-ELECTROCHEMICAL DETECTION; TANDEM MASS-SPECTROMETRY; MOLECULAR RECOGNITION; OXIDATIVE STRESS; RNA MOLECULES; URINE SAMPLES; 3RD STRAND; BASE; QUANTIFICATION; 8-OXODG;
D O I：
10.1021/ac3033323
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
8-Oxo-7,8-dihydroguanine (8-oxoG, or OG) as a free base has been widely considered as a biomarker for DNA oxidative damage. Currently no fluorescence sensor has been developed to directly detect 8-oxoG less than 100 nM. In this study, two triple-stranded DNAs were selected as the scaffolds to rationally design DNA aptamer sensors for 8-oxoG. The cavity was created by deleting the 8-oxodG nucleoside in a triplex containing an A.OG-C triad or a C-OG-A triad. The results showed that the fluorescence of both sensors were completely quenched by 8-oxoG. The detection ranges of the two sensors were different, while the combined range was comparable to the detection range of an antibody-based method. This result is expected to enable a fast, low-cost, and reusable method to measure 8-oxoG concentration.
引用
收藏
页码：201 / 207
页数：7
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