Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods:: resazurin and nitrate reductase assays

被引：15
|
作者：
Affolabi, Dissou [1 ,2 ]
Sanoussi, N'Dira [1 ]
Odoun, Mathieu [1 ]
Martin, Anandi [2 ,3 ]
Koukpemedji, Louis [1 ]
Palomino, Juan Carlos [2 ]
Kestens, Luc [4 ]
Anagonou, Severin [1 ]
Portaels, Francoise [2 ]
机构：
[1] Lab Reference Mycobacteries, Cotonou, Benin
[2] Inst Trop Med, Mycobacteriol Unit, B-2000 Antwerp, Belgium
[3] Med Sans Frontieres, F-75011 Paris, France
[4] Inst Trop Med, Immunol Unit, B-2000 Antwerp, Belgium
来源：
JOURNAL OF MEDICAL MICROBIOLOGY | 2008年 / 57卷 / 08期
关键词：
D O I：
10.1099/jmm.0.2008/000406-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We have evaluated two simple, rapid and low-cost colorimetric methods for the detection of multidrug-resistant Mycobacterium tuberculosis. A total of 151 M. tuberculosis strains were tested for resistance to rifampicin (RMP) and isoniazid by resazurin microplate assay (REMA) and nitrate reductase assay (NRA) in comparison with the conventional proportion method (PM) on Lowenstein-Jensen medium. A complete agreement was found between NRA and PM, while one false RMP-susceptible result was found by REMA. REMA and NRA tests are rapid and inexpensive, and could be good alternatives to the conventional PM in low-resource countries.
引用
收藏
页码：1024 / 1027
页数：4
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