Cytogenetic and Molecular Characterization of B-Genome Introgression Lines of Brassica napus L.

被引:12
|
作者
Dhaliwal, Inderpreet [1 ]
Mason, Annaliese S. [2 ,3 ]
Banga, Shashi [1 ]
Bharti, Sakshi [1 ]
Kaur, Beerpal [1 ]
Gurung, Allison Mary [5 ]
Salisbury, Phillip Anthony [5 ]
Batley, Jacqueline [2 ,4 ]
Banga, Surinder Singh [1 ]
机构
[1] Punjab Agr Univ, Dept Genet & Plant Breeding, Ludhiana 141001, Punjab, India
[2] Univ Queensland, Sch Agr & Food Sci, Brisbane, Qld 4072, Australia
[3] Justus Liebig Univ, Dept Plant Breeding, Res Ctr Biosyst Land Use & Nutr, D-35392 Giessen, Germany
[4] Univ Western Australia, Sch Plant Biol, Crawley, WA 6009, Australia
[5] Univ Melbourne, Inst Land & Food Resources, Parkville, Vic 3010, Australia
来源
G3-GENES GENOMES GENETICS | 2017年 / 7卷 / 01期
基金
澳大利亚研究理事会;
关键词
oilseed rape; siliqua shatter resistance; fl-GISH; graphical genotyping; SNP genotyping; QUANTITATIVE TRAIT LOCI; NUCLEAR-DNA CONTENT; GENETIC DIVERSITY; ARABIDOPSIS-THALIANA; RAPESEED; JUNCEA; HETEROSIS; MARKERS; CANOLA; YIELD;
D O I
10.1534/g3.116.036442
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Brassica napus introgression lines (ILs), having B-genome segments from B. carinata, were assessed genetically for extent of introgression and phenotypically for siliqua shatter resistance. Introgression lines had 7-9% higher DNA content, were meiotically stable, and had almost normal pollen fertility/seed set. Segment introgressions were confirmed by fluorescent genomic in situ hybridization (fl-GISH), SSR analyses, and SNP studies. Genotyping with 48 B-genome specific SSRs detected substitutions from B3, B4, B6, and B7 chromosomes on 39 of the 69 ILs whereas SNP genotyping detected a total of 23 B-segments (>= 3 Mb) from B4, B6, and B7 introgressed into 10 of the 19 (C1, C2, C3, C5, C6, C8, C9, A3, A9, A10) chromosomes in 17 ILs. The size of substitutions varied from 3.0 Mb on chromosome A9 (IL59) to 42.44 Mb on chromosome C2 (IL54), ranging from 7 to 83% of the recipient chromosome. Average siliqua strength in ILs was observed to be higher than that of B. napus parents (2.2-6.0 vs. 1.9-4.0 mJ) while siliqua strength in some of the lines was almost equal to that of the donor parent B. carinata (6.0 vs. 7.2 mJ). These ILs, with large chunks of substituted B-genome, can prove to be a useful prebreeding resource for germplasm enhancement in B. napus, especially for siliqua shatter resistance.
引用
收藏
页码:77 / 86
页数:10
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