Identification of a functional envelope protein from the HERV-K family of human endogenous retroviruses

被引:105
作者
Dewannieux, M [1 ]
Blaise, S [1 ]
Heidmann, T [1 ]
机构
[1] Inst Gustave Roussy, CNRS, UMR 8122, Unite Retrovirus Endogenes & Elements Retroides E, F-94805 Villejuif, France
关键词
D O I
10.1128/JVI.79.24.15573-15577.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Genome-wide screening of sequence databases for human endogenous retroviruses (HERVs) has led to the identification of 18 coding env genes, among which two-the syncytin genes-encode fusogenic ENV proteins possibly involved in placenta physiology. Here we show that a third ENV, originating from the most "recent" HERV-K(HML2) family, is functional. Immunofluorescence analysis of env-transduced cells demonstrates expression of the protein at the cell surface, and we show that the protein confers infectivity to simian immunodeficiency virus pseudotypes. Western blot analysis of the pseudotyped virions further discloses the expected specific cleavage of the ENV precursor protein. This functional ENV could play a role in the amplification-via infection of the germ line-of the HERV-K genomic copies, all the more as coding HERV-K gag and pol genes can similarly be found in the human genome, which could therefore generate infectious virions of a fully endogenous origin.
引用
收藏
页码:15573 / 15577
页数:5
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