Affinity-Based Labeling of Cytohesins with a Bifunctional SecinH3 Photoaffinity Probe

被引：22
|
作者：
Bi, Xihe [1 ]
Schmitz, Anton [1 ]
Hayallah, Alaa M. [1 ]
Song, Jin-Na [1 ]
Famulok, Michael [1 ]
机构：
[1] Univ Bonn, LIMES Inst Program Unit Chem Biol & Med Chem, D-53121 Bonn, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 49期
关键词：
benzophenone; cytohesins; inhibitors; photoaffinity labeling; proteins;
D O I：
10.1002/anie.200803962
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
(Chemical Equation Presented) The specific binding of the cytohesin inhibitor SecinH3 to Sec7 domains of cytohesins was shown by the use of a bifunctional photoaffinity probe (see structure) on guanine nucleotide exchange factors (GEFs) and GTPases. This probe should be useful for the proteome-wide profiling of cytohesin complexes and the identification of the binding site. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
引用
收藏
页码：9565 / 9568
页数：4
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