A disposable immunosensor for Enterobacter sakazakii based on an electrochemically reduced graphene oxide-modified electrode

被引：19
|
作者：
Hu, Xue [1 ]
Dou, Wenchao [1 ]
Fu, Linglin [1 ]
Zhao, Guangying [1 ]
机构：
[1] Zhejiang Gongshang Univ, Dept Food Qual & Safety, Food Safety Key Lab Zhejiang Prov, Hangzhou 310035, Zhejiang, Peoples R China
来源：
ANALYTICAL BIOCHEMISTRY | 2013年 / 434卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Electrochemically reduced graphene oxide; Immunosensor; Enterobacter sakazakii; PCR;
D O I：
10.1016/j.ab.2012.11.013
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A disposable immunosensor based on electrochemically reduced graphene oxide (ERGO) was developed for the detection of Enterobacter sakazakii. First, the graphene was deposited on a screen-printed carbon electrode (SPCE) by an electrochemical method. Second, the horseradish peroxidase-labeled bacteria-specific antibody was assembled onto the modified electrode to enhance the sensitivity of the immunosensor. The immunosensor constructed in this study can sensitively and rapidly detect E. sakazakii, and this method may contribute to further foodborne pathogen detection. In addition, this ERGO-modified SPCE could also provide new insights into the development of immunosensors for other bacteria to facilitate rapid detection. (c) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码：218 / 220
页数：3
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