Crystal structure of plant pectin methylesterase

被引：111
|
作者：
Johansson, K
El-Ahmad, M
Friemann, R
Jörnvall, H
Markovic, O
Eklund, H [1 ]
机构：
[1] Swedish Univ Agr Sci, Dept Biol Mol, S-75124 Uppsala, Sweden
[2] Karolinska Inst, Dept Med Biochem & Biophys, S-17177 Stockholm, Sweden
[3] Slovak Acad Sci, Inst Chem, Bratislava 84238, Slovakia
来源：
FEBS LETTERS | 2002年 / 514卷 / 2-3期
关键词：
crystal structure; beta-helix; pectin methylesterase; plant enzyme; catalytic mechanism; binding cleft;
D O I：
10.1016/S0014-5793(02)02372-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Pectin is a principal component in the primary cell wall of plants. During cell development, pectin is modified by pectin methylesterases to give different properties to the cell wall. This report describes the first crystal structure of a plant pectin methylesterase. The P-helical structure embodies a central cleft, lined by several aromatic residues, that has been deduced to be suitable for pectin binding. The active site is found at the center of this cleft where Asp157 is suggested to act as the nucleophile, Asp136 as an acid/base and Gln113/Gln135 to form an anion hole to stabilize the transition state. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.
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收藏
页码：243 / 249
页数：7
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