Thermo-optical characterization of fluorescent rhodamine B based temperature-sensitive nanosensors using a CMOS MEMS micro-hotplate

被引：47

作者：

Chauhan, Veeren M. ^{[1
]}

Hopper, Richard H. ^{[2
]}

Ali, Syed Z. ^{[2
]}

King, Emma M. ^{[3
]}

Udrea, Florin ^{[2
,4
]}

Oxley, Chris H. ^{[5
]}

Aylott, Jonathan W. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Pharm, Lab Biophys & Surface Anal, Nottingham NG7 2RD, England

[2] Cambridge CMOS Sensors, Cambridge CB2 3BZ, England

[3] Univ Nottingham, Queens Med Ctr, Sch Biomed Sci, Adv Microscopy Unit, Nottingham NG7 2UH, England

[4] Univ Cambridge, Dept Engn, Elect Engn Div, Cambridge CB3 0FA, England

[5] De Montfort Univ, Fac Technol, Leicester LE1 9BH, Leics, England

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 192卷

基金：

英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

MEMS micro hotplate; Fluorescent; Temperature-sensitive; Nanosensor; Rhodamine B; Silica sol-gel; DYNAMIC-RANGE; HOT-STAGE; RESOLUTION; GROWTH; GEL; PH;

D O I：

10.1016/j.snb.2013.10.042

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A custom designed microelectromechanical systems (MEMS) micro-hotplate, capable of operating at high temperatures (up to 700 degrees C), was used to thermo-optically characterize fluorescent temperature-sensitive nanosensors. The nanosensors, 550 nm in diameter, are composed of temperature-sensitive rhodamine B (RhB) fluorophore which was conjugated to an inert silica sol-gel matrix. Temperature-sensitive nanosensors were dispersed and dried across the surface of the MEMS micro-hotplate, which was mounted in the slide holder of a fluorescence confocal microscope. Through electrical control of the MEMS micro-hotplate, temperature induced changes in fluorescence intensity of the nanosensors was measured over a wide temperature range. The fluorescence response of all nanosensors dispersed across the surface of the MEMS device was found to decrease in an exponential manner by 94%, when the temperature was increased from 25 degrees C to 145 degrees C. The fluorescence response of all dispersed nanosensors across the whole surface of the MEMS device and individual nanosensors, using line profile analysis, were not statistically different (p<0.05). The MEMS device used for this study could prove to be a reliable, low cost, low power and high temperature micro-hotplate for the thermo-optical characterisation of sub-micron sized particles. The temperature-sensitive nanosensors could find potential application in the measurement of temperature in biological and micro-electrical systems. (C) 2013 The Authors. Published by Elsevier B.V. All rights reserved.

引用

页码：126 / 133

页数：8