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Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape strips
被引:3
|作者:
Multari, Dylan H.
[1
]
Ravishankar, Prathiba
[1
]
Sullivan, Geraldine J.
[1
]
Power, Ronika K.
[2
,3
,4
]
Lord, Constance
[5
]
Fraser, James A.
[6
]
Haynes, Paul A.
[1
,3
,4
]
机构:
[1] Macquarie Univ, Sch Nat Sci, N Ryde, NSW 2109, Australia
[2] Macquarie Univ, Dept Hist & Archaeol, N Ryde, NSW 2109, Australia
[3] Macquarie Univ, Biomol Discovery Res Ctr, N Ryde, NSW 2109, Australia
[4] Macquarie Univ, Ctr Ancient Cultural Heritage & Environm, N Ryde, NSW 2109, Australia
[5] Univ Sydney, Chau Chak Wing Museum, Camperdown, NSW 2006, Australia
[6] British Museum, Middle East Dept, London, England
来源:
基金:
澳大利亚研究理事会;
关键词:
Paleoproteomics;
Deamidation;
Bioarchaeology;
Cultural heritage;
Mass spectrometry;
1 @MQNatSci;
D O I:
10.1016/j.dib.2022.108562
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified hu-man remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sam-pling tape strips. A collection of cranial and other bone frag-ments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of < 1%. Deamidation ratios were as-sessed using an in-house R script, with asparagine deamida-tion averaging-20-30% and glutamine deamidation averag-ing-15-25%.(c) 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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