Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape strips

被引:3
|
作者
Multari, Dylan H. [1 ]
Ravishankar, Prathiba [1 ]
Sullivan, Geraldine J. [1 ]
Power, Ronika K. [2 ,3 ,4 ]
Lord, Constance [5 ]
Fraser, James A. [6 ]
Haynes, Paul A. [1 ,3 ,4 ]
机构
[1] Macquarie Univ, Sch Nat Sci, N Ryde, NSW 2109, Australia
[2] Macquarie Univ, Dept Hist & Archaeol, N Ryde, NSW 2109, Australia
[3] Macquarie Univ, Biomol Discovery Res Ctr, N Ryde, NSW 2109, Australia
[4] Macquarie Univ, Ctr Ancient Cultural Heritage & Environm, N Ryde, NSW 2109, Australia
[5] Univ Sydney, Chau Chak Wing Museum, Camperdown, NSW 2006, Australia
[6] British Museum, Middle East Dept, London, England
来源
DATA IN BRIEF | 2022年 / 45卷
基金
澳大利亚研究理事会;
关键词
Paleoproteomics; Deamidation; Bioarchaeology; Cultural heritage; Mass spectrometry; 1 @MQNatSci;
D O I
10.1016/j.dib.2022.108562
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified hu-man remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sam-pling tape strips. A collection of cranial and other bone frag-ments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of < 1%. Deamidation ratios were as-sessed using an in-house R script, with asparagine deamida-tion averaging-20-30% and glutamine deamidation averag-ing-15-25%.(c) 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )
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页数:7
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  • [1] Development of a novel minimally invasive sampling and analysis technique using skin sampling tape strips for bioarchaeological proteomics
    Multari, Dylan H.
    Ravishankar, Prathiba
    Sullivan, Geraldine J.
    Power, Ronika K.
    Lord, Constance
    Fraser, James A.
    Haynes, Paul A.
    JOURNAL OF ARCHAEOLOGICAL SCIENCE, 2022, 139