Initial crystallographic study of human PCNA in complex with a peptide containing the noncanonical PIP-box sequence of human DNA polymerase ι

被引：5

作者：

Hishiki, Asami ^{[1
]}

Shimizu, Toshiyuki ^{[1
]}

Hanafusa, Tomo ^{[2
]}

Ohmori, Haruo ^{[2
]}

Sato, Mamoru ^{[1
]}

Hashimoto, Hiroshi ^{[1
]}

机构：

[1] Yokohama City Univ, Int Grad Sch Arts & Sci, Yokohama, Kanagawa, Japan

[2] Kyoto Univ, Inst Virus Res, Kyoto 6068501, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2008年 / 64卷

基金：

日本学术振兴会;

关键词：

D O I：

10.1107/S1744309108028522

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human DNA polymerase iota (Pol iota) is one of the Y-family DNA polymerases involved in translesion synthesis (TLS), which allows continued replication at damaged DNA templates. Pol iota has a noncanonical PCNA-interacting protein box (PIP-box) within an internal region of the protein. Pol iota activity is stimulated by PCNA binding through the noncanonical PIP-box. To clarify the interaction of PCNA with the noncanonical PIP-box of Pol iota, PCNA and a Pol iota peptide carrying the noncanonical PIP-box complex have been cocrystallized. The crystal belongs to space group C2, with unit-cell parameters a = 167.1, b = 68.7, c = 90.0 angstrom, beta = 95.1 degrees. Structural analysis by molecular replacement is in progress.

引用

页码：954 / 956

页数：3

共 15 条

[1] Unlocking the PIP-box: A peptide library reveals interactions that drive high-affinity binding to human PCNA
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Dixon, Nicholas E.
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[3] Crystal structure of human PCNA in complex with the PIP box of DVC1
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Ohashi, E
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