Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A

被引：15

作者：

He, Jianlong ^{[1
]}

Sakaguchi, Kouta ^{[1
]}

Suzuki, Tohru ^{[1
]}

机构：

[1] Gifu Univ, United Grad Sch Agr Sci, Gifu 5011193, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2012年 / 113卷 / 04期

关键词：

RBS; Bifidobacterium; Shine-Dalgarno sequence; Translation; ESCHERICHIA-COLI; TRANSLATION INITIATION; GENE; THERAPY; CLONING;

D O I：

10.1016/j.jbiosc.2011.11.019

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5 nt spacer length produced the most efficient protein expression. (C) 2011, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：442 / 444

页数：3

共 3 条

[1] CORRELATION BETWEEN A COAT PROTEIN AMINO-TERMINAL SEQUENCE AND A RIBOSOME-BINDING DNA SEQUENCE FROM PHI-X 174
AIR, GM
BRIDGEN, J
NATURE-NEW BIOLOGY, 1973, 241 (106): : 40 - 41
[2] A RIBOSOME BINDING-SITE SEQUENCE IS NECESSARY FOR EFFICIENT EXPRESSION OF THE DISTAL GENE OF A TRANSLATIONALLY-COUPLED GENE PAIR
DAS, A
YANOFSKY, C
NUCLEIC ACIDS RESEARCH, 1984, 12 (11) : 4757 - 4768
[3] Replication of poliovirus requires binding of the poly(rC) binding protein to the cloverleaf as well as to the adjacent C-rich spacer sequence between the cloverleaf and the internal ribosomal entry site
Toyoda, Hidemi
Franco, David
Fujita, Kentaro
Paul, Aniko V.
Wimmer, Eckard
JOURNAL OF VIROLOGY, 2007, 81 (18) : 10017 - 10028

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