Development of quantitative mass spectrometric immunoassay for serum amyloid A

被引：20

作者：

Trenchevska, Olgica ^{[1
]}

Yassine, Hussein N. ^{[2
]}

Borges, Chad R. ^{[1
]}

Nelson, Randall W. ^{[1
]}

Nedelkov, Dobrin ^{[1
]}

机构：

[1] Arizona State Univ, Biodesign Inst, 727 E Tyler St, Tempe, AZ 85287 USA

[2] Univ Southern Calif, Dept Med, Los Angeles, CA USA

来源：

BIOMARKERS | 2016年 / 21卷 / 08期

关键词：

Baseline concentration; inflammation biomarker; mass spectrometry; polymorphism; posttranslational modifications; proteoforms; C-REACTIVE PROTEIN; CORONARY-ARTERY-DISEASE; SYSTEMIC AA-AMYLOIDOSIS; ACUTE-PHASE PROTEIN; HUMAN-PLASMA; GENE FAMILY; SAA; CHOLESTEROL; ISOFORMS; VARIANTS;

D O I：

10.1080/1354750X.2016.1201533

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Objective: Proteins can exist as multiple proteoforms in vivo that can have important roles in physiological and pathological states. Methods: We present the development and characterization of mass spectrometric immunoassay (MSIA) for quantitative determination of serum amyloid A (SAA) proteoforms. Results: Intra-and inter-day precision revealed CVs <10%. Against existing SAA ELISA, the developed MSIA showed good correlation according to the Altman-Bland plot. Individual concentrations of the SAA proteoforms across a cohort of 170 samples revealed 7 diverse SAA polymorphic types and 12 different proteoforms. Conclusion: The new SAA MSIA enables parallel analysis of SAA polymorphisms and quantification of all expressed SAA proteoforms, in a high-throughput and time-efficient manner.

引用

页码：743 / 751

页数：9

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