Development of 12 microsatellite markers for Aconitum brachypodum (Ranunculaceae), a critically endangered and endemic medicinal plant

被引：1

作者：

Li, Ya-Qiong ^{[1
]}

Meng, Yi ^{[2
]}

Zhang, Jie ^{[1
]}

机构：

[1] Yunnan Univ TCM, Coll Pharmacol, Kunming 650500, Peoples R China

[2] Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650201, Peoples R China

来源：

BIOCHEMICAL SYSTEMATICS AND ECOLOGY | 2015年 / 61卷

基金：

中国国家自然科学基金;

关键词：

Aconitum brachypodum; Microsatellite markers; Genetics; Critically endangered; Medicinal plant;

D O I：

10.1016/j.bse.2015.06.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, 12 microsatellite markers were developed from two microsatellite-enriched libraries (AG, AC) of Aconitum brachypodum, which were constructed using a FIASCO method. Polymorphism of each locus was assessed in 24 individuals of A. brachypodum. Number of alleles per locus (N-A) ranged from 2 to 4. The average allele number of the microsatellites was 2.58 per locus. The observed (H-O) and expected (H-E) heterozygosities ranged from 0.125 to 0.875 and 0.117 to 0.612, respectively. Polymorphic information content (PLC) ranged from 0.110 to 0.531. Among the 12 microsatellite markers, three deviated from Hardy-Weinberg equilibrium significantly. These markers will facilitate further studies on the population genetics of A. brachypodum. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：462 / 464

页数：3

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