Development and Validation of a Reversed-Phase Liquid Chromatography Method for the Simultaneous Determination of Indole-3-Acetic Acid, Indole-3-Pyruvic Acid, and Abscisic Acid in Barley (Hordeum vulgare L.)

被引:37
|
作者
Nakurte, Ilva [1 ]
Keisa, Anete [1 ]
Rostoks, Nils [1 ]
机构
[1] Univ Latvia, Fac Biol, LV-1586 Riga, Latvia
关键词
PLANT DEVELOPMENT; AUXIN; CYTOKININS; SEPARATION; GROWTH;
D O I
10.1155/2012/103575
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple, sensitive, precise, and specific reverse HPLC method was developed and validated for the determination of plant hormones in barley (Hordeum vulgare L.). The method includes extraction in aqueous organic solvent followed by solid-phase extraction, sample evaporation, and reversed-phase HPLC analysis in a general purpose UV-visible (abscisic acid (ABA)) and fluorescence detection (indole-3-acetic acid (IAA) and indole-3-pyruvic acid (IPA)), high-performance liquid chromatography system. The separation was carried out on Zorbax Eclipse XDB C8 column (150 x 4.6 mm I. D) with a mobile phase composed of methanol and 1% acetic acid (60 : 40 v/v) in isocratic mode at a flow rate of 1 ml min(-1). The detection was monitored at 270 nm (ABA) and at 282 nm (Ex) and 360 nm (Em) (IAA, IPA). The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantification, and robustness. The determined validation parameters are in the commonly acceptable ranges for that kind of analysis.
引用
收藏
页数:6
相关论文
共 50 条
  • [21] STUDIES ON OXIDATION OF INDOLE-3-ACETIC ACID BY PEROXIDASE ENZYMES .I. COLORIMETRIC DETERMINATION OF INDOLE-3-ACETIC ACID OXIDATION PRODUCTS
    MEUDT, WJ
    GAINES, TP
    PLANT PHYSIOLOGY, 1967, 42 (10) : 1395 - &
  • [22] Indole-3-acetic acid, ethylene, and abscisic acid metabolism in developing muskmelon (Cucumis melo L) fruit
    Dunlap, JR
    Slovin, JP
    Cohen, JD
    PLANT GROWTH REGULATION, 1996, 19 (01) : 45 - 54
  • [23] Determination of Indole-3-acetic Acid and Abscisic Acid by Double-Direction Dispersive Liquid-Liquid Microextraction Coupled with High-Performance Liquid Chromatography
    Hao, Liangcheng
    Wang, Yingying
    Guo, Suping
    Qi, Guohui
    Zhang, Xuemei
    Li, Baoguo
    ANALYTICAL LETTERS, 2015, 48 (04) : 586 - 593
  • [24] Indole-3-Acetic Acid Synthesis by Plant Growth Promoting Klebsiella sp. (PGPB1) via Indole-3-Pyruvic Acid Pathway and its Uptake in Plants
    Alvarez, Paul Lloydson J.
    Merca, Florinia E.
    Fernando, Lilia M.
    Rallos, Roland V.
    Mendoza, Christopher O.
    PHILIPPINE JOURNAL OF CROP SCIENCE, 2019, 44 (03): : 1 - 9
  • [25] Role of Brassinosteroids, Ethylene, Abscisic Acid, and Indole-3-Acetic Acid in Mango Fruit Ripening
    Sakimin S. Zaharah
    Zora Singh
    Gregory M. Symons
    James B. Reid
    Journal of Plant Growth Regulation, 2012, 31 : 363 - 372
  • [26] Role of Brassinosteroids, Ethylene, Abscisic Acid, and Indole-3-Acetic Acid in Mango Fruit Ripening
    Zaharah, Sakimin S.
    Singh, Zora
    Symons, Gregory M.
    Reid, James B.
    JOURNAL OF PLANT GROWTH REGULATION, 2012, 31 (03) : 363 - 372
  • [27] Different behaviour of indole-3-acetic acid and indole-3-butyric acid in stimulating lateral root development in rice (Oryza sativa L.)
    Chhun, T
    Taketa, S
    Tsurumi, S
    Ichii, M
    PLANT GROWTH REGULATION, 2004, 43 (02) : 135 - 143
  • [28] Different Behaviour of Indole-3-Acetic Acid and Indole-3-Butyric Acid in Stimulating Lateral Root Development in Rice (Oryza sativa L.)
    Tory Chhun
    Shin Taketa
    Seiji Tsurumi
    Masahiko Ichii
    Plant Growth Regulation, 2004, 43 : 135 - 143
  • [29] Definitive identification of indole-3-acetic acid and abscisic acid in shoots of Coleus blumei by gas chromatography mass spectrometry
    LaMotte, CE
    Li, XY
    Jacobs, WP
    PLANT GROWTH REGULATION, 1998, 25 (03) : 201 - 203
  • [30] DOWEX-50 CHROMATOGRAPHY OF INDOLE-3-ACETIC ACID ESTERS
    EHMANN, A
    BANDURSK.RS
    PLANT PHYSIOLOGY, 1971, 47 : 3 - &