Design and quality control of short interfering RNA

被引：3
|
作者：
Grinev, V. V. [1 ]
机构：
[1] Belarusian State Univ, Fac Biol, Dept Genet, Minsk 220030, BELARUS
来源：
MOLECULAR BIOLOGY | 2012年 / 46卷 / 06期
关键词：
RNA interference; short interfering RNA; design rules; assessment of activity; assessment of specificity; INFLUENCES SIRNA EFFICACY; SILENCING EFFICIENCY; SECONDARY STRUCTURE; GENE-EXPRESSION; MESSENGER-RNA; DEPENDENT STIMULATION; CHEMICAL-MODIFICATION; SYSTEMATIC ANALYSIS; MAMMALIAN-CELLS; RATIONAL DESIGN;
D O I：
10.1134/S0026893312050056
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This review is devoted to analyzing design rules, as well as quality controls, in the development of highly effective and specific short interfering RNA (siRNA). Four crucial steps in the development of this siRNA are discussed, i.e., selecting target RNA and designing sense and antisense strands, as well as assessing the activity and specificity of corresponding siRNA. Special consideration is given to the principles of siRNA construction based on both structural and thermodynamical features and the nucleotide composition of siRNA, as well as structural and thermodynamical properties of target RNA and features of experimental performance. Bioinformatics resources for developing siRNA are also discussed. Information from this review can be useful for the development of highly effective and specific siRNA, short hairpin RNA (shRNA), and/or artificial microRNA (amiRNA) sequences for experimental gene therapy and functional genomics.
引用
收藏
页码：739 / 754
页数：16
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