Two-Photon Imaging of Cellular Activities in Oxygen Sensing Tissues

被引：2
|
作者：
Wotzlaw, Christoph [1 ]
Berchner-Pfannschmidt, Utta [1 ]
Fandrey, Joachim [1 ]
Acker, Helmut [1 ]
机构：
[1] Univ Duisburg Essen, Inst Physiol, D-45122 Essen, Germany
来源：
MICROSCOPY AND MICROANALYSIS | 2008年 / 14卷 / 06期
关键词：
oxygen sensing; HIF; membrane potential; (Ca++)(i); U2OS cell; carotid body; kidney; FRET; FRAP; 3D imaging;
D O I：
10.1017/S1431927608080847
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The cellular oxygen sensing system of the body ensures appropriate adaptation of cellular functions toward hypoxia by regulating gene expression and ion channel activity. Two-photon laser microscopy is an ideal tool to study and prove the relevance of the molecular mechanisms within oxygen sensing pathways on the cellular and complex tissue or organ level. Images of hypoxia inducible factor 1 (HIF-1) subunit nuclear mobility and protein-protein interaction in living cells, of hypoxia-induced changes in membrane potential and intracellular calcium of live ex vivo carotid bodies as well as of rat kidney proximal tubulus function in vivo, will be shown.
引用
收藏
页码：519 / 525
页数：7
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