Veronica austriaca L. Extract and Arbutin Expand Mature Double TNF-α/IFN-γ Neutrophils in Murine Bone Marrow Pool

被引：2

作者：

Dimitrova, Petya A. ^{[1
]}

Alipieva, Kalina ^{[2
]}

Grozdanova, Tsvetinka ^{[2
]}

Leseva, Milena ^{[1
]}

Gerginova, Dessislava ^{[2
]}

Simova, Svetlana ^{[2
]}

Marchev, Andrey S. ^{[3
]}

Bankova, Vassya ^{[2
]}

Georgiev, Milen, I ^{[3
]}

Popova, Milena P. ^{[2
]}

机构：

[1] Bulgarian Acad Sci, Stefan Angeloff Inst Microbiol, Dept Immunol, Bl 26 Acad Georgi Bonchev Str, Sofia 1113, Bulgaria

[2] Bulgarian Acad Sci, Inst Organ Chem, Ctr Phytochem, Bl 9 Acad Georgi Bonchev Str, Sofia 1113, Bulgaria

[3] Bulgarian Acad Sci, Stephan Angeloff Inst Microbiol, Lab Metabol, 139 Ruski Blvd, Plovdiv 4000, Bulgaria

来源：

MOLECULES | 2020年 / 25卷 / 15期

关键词：

Veronica austriaca; arbutin; neutrophils; TNF-alpha; IFN-gamma; cell maturation; CHEMICAL MARKERS; ACTIVATION; IRIDOIDS; PLANTS; CELLS;

D O I：

10.3390/molecules25153410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants from theVeronicagenus are used across the world as traditional remedies. In the present study, extracts from the aerial part of the scarcely investigatedVeronica austriacaL., collected from two habitats in Bulgaria-the Balkan Mountains (Vau-1) and the Rhodopi Mountains (Vau-2), were analyzedbynuclear magnetic resonance (NMR) spectroscopy. The secondary metabolite, arbutin, was identified as a major constituent in both extracts, and further quantified by high-performance liquid chromatography (HPLC), while catalpol, aucubin and verbascoside were detected at lower amounts. The effect of the extracts and of pure arbutin on the survival of neutrophils isolated from murine bone marrow (BM) were determined by colorimetric assay. The production of cytokines-tumor necrosis factor (TNF)-alpha and interferon (IFN)-gamma was evaluated by flowcytometry. While Vau-1 inhibited neutrophil vitality in a dose-dependent manner, arbutin stimulated the survival of neutrophils at lower concentrations, and inhibited cell density at higher concentrations. The Vau-1 increased the level of intracellular TNF-alpha, while Vau-2 and arbutin failed to do so, and expanded the frequency of mature double TNF-alpha(+)/IFN-gamma(hi)neutrophils within the BM pool.

引用

页数：15