PsAP2 an AP2/ERF family transcription factor from Papaver somniferum enhances abiotic and biotic stress tolerance in transgenic tobacco

被引:49
|
作者
Mishra, Sonal [1 ]
Phukan, Ujjal J. [1 ]
Tripathi, Vineeta [2 ]
Singh, Dhananjay K. [1 ]
Luqman, Suaib [1 ]
Shukla, Rakesh Kumar [1 ]
机构
[1] Cent Inst Med & Aromat Plants CSIR CIMAP, Div Biotechnol, Lucknow 226015, Uttar Pradesh, India
[2] CSIR, Cent Drug Res Inst, Bot Div, Lucknow 226031, Uttar Pradesh, India
关键词
AP2/ERF; Abiotic and biotic stress; AOX1a; Papaver somniferum; Reactive oxygen species; OXIDATIVE STRESS; GENE; PROTEIN; DEHYDRATION; EXPRESSION; RESPONSES; BINDING; SALT; OVEREXPRESSION; RESISTANCE;
D O I
10.1007/s11103-015-0361-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The AP2/ERFs are one of the most important family of transcription factors which regulate multiple responses like stress, metabolism and development in plants. We isolated PsAP2 a novel AP2/ERF from Papaver somniferum which was highly upregulated in response to wounding followed by ethylene, methyl jasmonate and ABA treatment. PsAP2 showed specific binding with both DRE and GCC box elements and it was able to transactivate the reporter genes in yeast. PsAP2 overexpressing transgenic tobacco plants exhibited enhanced tolerance towards both abiotic and biotic stresses . Real time transcript expression analysis showed constitutive upregulation of tobacco Alternative oxidase1a and Myo-inositol-1-phosphate synthase in PsAP2 overexpressing tobacco plants. Further, PsAP2 showed interaction with NtAOX1a promoter in vitro, it also specifically activated the NtAOX1a promoter in yeast and tobacco BY2 cells. The silencing of PsAP2 using VIGS lead to significant reduction in the AOX1 level in P. somniferum. Taken together PsAP2 can directly bind and transcriptionally activate NtAOX1a and its overexpression in tobacco imparted increased tolerance towards both abiotic and biotic stress.
引用
收藏
页码:173 / 186
页数:14
相关论文
共 50 条
  • [1] PsAP2 an AP2/ERF family transcription factor from Papaver somniferum enhances abiotic and biotic stress tolerance in transgenic tobacco
    Sonal Mishra
    Ujjal J. Phukan
    Vineeta Tripathi
    Dhananjay K. Singh
    Suaib Luqman
    Rakesh Kumar Shukla
    Plant Molecular Biology, 2015, 89 : 173 - 186
  • [2] AP2/ERF Transcription Factors for Tolerance to Both Biotic and Abiotic Stress Factors in Plants
    Shuming Nie
    Dan Wang
    Tropical Plant Biology, 2023, 16 : 105 - 112
  • [3] AP2/ERF Transcription Factors for Tolerance to Both Biotic and Abiotic Stress Factors in Plants
    Nie, Shuming
    Wang, Dan
    TROPICAL PLANT BIOLOGY, 2023, 16 (03) : 105 - 112
  • [4] Overexpression of an AP2/ERF Type Transcription Factor OsEREBP1 Confers Biotic and Abiotic Stress Tolerance in Rice
    Jisha, V.
    Dampanaboina, Lavanya
    Vadassery, Jyothilakshmi
    Mithoefer, Axel
    Kappara, Saivishnupriya
    Ramanan, Rajeshwari
    PLOS ONE, 2015, 10 (06):
  • [5] AP2/ERF family transcription factors in plant abiotic stress responses
    Mizoi, Junya
    Shinozaki, Kazuo
    Yamaguchi-Shinozaki, Kazuko
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, 2012, 1819 (02): : 86 - 96
  • [6] Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis
    Xiong, Ai-Sheng
    Jiang, Hai-Hua
    Zhuang, Jing
    Peng, Ri-He
    Jin, Xiao-Fen
    Zhu, Bo
    Wang, Feng
    Zhang, Jian
    Yao, Quan-Hong
    MOLECULAR BIOTECHNOLOGY, 2013, 53 (02) : 198 - 206
  • [7] Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis
    Ai-Sheng Xiong
    Hai-Hua Jiang
    Jing Zhuang
    Ri-He Peng
    Xiao-Fen Jin
    Bo Zhu
    Feng Wang
    Jian Zhang
    Quan-Hong Yao
    Molecular Biotechnology, 2013, 53 : 198 - 206
  • [8] AP2/ERF Transcription Factor Regulatory Networks in Hormone and Abiotic Stress Responses in Arabidopsis
    Xie, Zhouli
    Nolan, Trevor M.
    Jiang, Hao
    Yin, Yanhai
    FRONTIERS IN PLANT SCIENCE, 2019, 10
  • [9] A New AP2/ERF Transcription Factor from the Oil Plant Jatropha curcas Confers Salt and Drought Tolerance to Transgenic Tobacco
    Wang, Xuehua
    Han, Haiyang
    Yan, Jun
    Chen, Fang
    Wei, Wei
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2015, 176 (02) : 582 - 597
  • [10] A New AP2/ERF Transcription Factor from the Oil Plant Jatropha curcas Confers Salt and Drought Tolerance to Transgenic Tobacco
    Xuehua Wang
    Haiyang Han
    Jun Yan
    Fang Chen
    Wei Wei
    Applied Biochemistry and Biotechnology, 2015, 176 : 582 - 597