Development and characterization of 16 microsatellite markers for Micromeria (Lamiaceae) from Tenerife (Canary Islands, Spain) using 454 sequencing

被引：4
|
作者：
Puppo, Pamela [1 ]
Curto, Manuel [1 ]
Meimberg, Harald [1 ,2 ]
机构：
[1] Univ Porto, InBio Associate Lab, Res Ctr Biodivers & Genet Resources, CIBIO, P-4485661 Vairao, Portugal
[2] Univ Nat Resources & Life Sci, Inst Integrat Nat Conservat Res, A-1180 Vienna, Austria
来源：
CONSERVATION GENETICS RESOURCES | 2015年 / 7卷 / 03期
关键词：
Lamiaceae; Micromeria; Microsatellites; 454; GS-FLX;
D O I：
10.1007/s12686-015-0451-5
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
Micromeria presents eight species in Tenerife, and from these, three are endangered or critically endangered. In this paper we use a 454 run from M. hyssopifolia to develop and characterize 16 new microsatellite markers and test them using 19 individuals from two populations of this species. The number of alleles per locus varied from 2 to 10 and observed heterozygosity ranged from 0.125 to 0.900. Most loci successfully amplified in the threatened species M. densiflora, M. glomerata and M. rivas-martinezii and were also polymorphic. These loci will be valuable tools for assessing the genetic diversity of these endangered species.
引用
收藏
页码：743 / 749
页数：7
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