Role of biofilms in the survival of Legionella pneumophila in a model potable-water system

被引:214
|
作者
Murga, R [1 ]
Forster, TS
Brown, E
Pruckler, JM
Fields, BS
Donlan, RM
机构
[1] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Healthcare Qual Promot, Epidemiol & Lab Branch, Atlanta, GA 30333 USA
[2] Ctr Dis Control & Prevent, Natl Ctr Infect Dis, Div Bacterial & Mycot Dis, Resp Dis Branch, Atlanta, GA 30333 USA
来源
MICROBIOLOGY-SGM | 2001年 / 147卷
关键词
Hartmannella vermiformis; protozoa; growth; ecology;
D O I
10.1099/00221287-147-11-3121
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Legionellae can infect and multiply intracellularly in both human phagocytic cells and protozoa. Growth of legionellae in the absence of protozoa has been documented only on complex laboratory media. The hypothesis upon which this study was based was that biofilm matrices, known to provide a habitat and a gradient of nutrients, might allow the survival and multiplication of legionellae outside a host cell. This study determined whether Legionella pneumophila can colonize and grow in biofilms with and without an association with Hartmannella vermiformis. The laboratory model used a rotating disc reactor at a retention time of 6(.)7 h to grow biofilms on stainless steel coupons. The biofilm was composed of Pseudomonas aeruginosa, Klebsiella pneumoniae and a Flavobacterium sp. The levels of L. pneumophila cells present in the biofilm were monitored for 15 d, with and without the presence of H. vermiformis, and it was found that, although unable to replicate in the absence of H. vermiformis, L. pneumophila was able to persist.
引用
收藏
页码:3121 / 3126
页数:6
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