Determination of Phospholipids from Ctenopharyngodon Idellus Muscle by Direct-injection Electrospray Ionization Tandem Mass Spectrometry

A method was developed for the determination of phospholipids from Ctenopharyngodon idellus muscle by direct-injection electrospray ionization tandem mass spectrometry (ESI-MS/MS). The samples were extracted with modified Bligh Dyer method and the crude extracts were taken directly into the electrospray ionization source by syringe pump. Under the precursor ion scan and neutral loss scan, intrasource separation and identification of six classes of phospholipids including phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl inositol, phosphatidyl serine, phosphatidyl glycerol and phosphatidic acid were achieved. The intensity of quasi-molecular ion of the phospholipids and concentration showed good linear relation in a certain range. The recoveries (67.1%-96.6%) and precision meet the requirements for analyzing biological samples. This method has been applied to analyze the molecular species and content of four class phospholipids from Ctenopharyngodon idellus muscle. The method is simple, fast accurate, and reproducibility with excellent stability, and suitable to analyze phospholipids from various biological samples for lipidomics research.