Construction of a doxycycline inducible adipogenic lentiviral expression system

被引：6

作者：

Liu, Q. ^{[1
]}

Hill, P. J. ^{[1
]}

Karamitri, A. ^{[1
,2
]}

Ryan, K. J. P. ^{[1
]}

Chen, H. Y. ^{[1
]}

Lomax, M. A. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Biosci, Loughborough LE12 5RD, Leics, England

[2] INSERM, U1016, Inst Cochin, Paris, France

来源：

PLASMID | 2013年 / 69卷 / 01期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Tetracycline inducible expression; Lentiviral plasmid; Adipose-specific promoter; Adipocyte; GENE-EXPRESSION; ADIPOCYTE DIFFERENTIATION; TRANSCRIPTIONAL COMPLEX; BROWN; CELLS; PROTEINS; ENHANCER; SWITCH;

D O I：

10.1016/j.plasmid.2012.10.001

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

To provide a tool for research on regulating adipocyte differentiation, tetracycline inducible (Tet on) lentiviral expression vectors under the control of an adipose-specific promoter were constructed. The lowest basal expression in the absence of doxycycline and most efficient dose-dependent, doxycycline-induced transient overexpression was observed using vectors constructed with a combination of Tetracycline Responsive Element (TRE) and reverse tetracycline-controlled TransActivator advanced (rtTAadv), transfected in white (3T3-L1) and brown (HIB-1B) preadipocytes cell lines. The results demonstrate that doxycycline adipogenic inducible expression can be achieved using a pLenti TRE / rtTA adv under the control of the truncated aP2 promoter in HIB-1B preadipocytes. (c) 2012 Elsevier Inc. All rights reserved.

引用

页码：96 / 103

页数：8

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