Use of Bordetella pertussis BP3385 To Establish a Cutoff Value for an IS481-Targeted Real-Time PCR Assay

被引：58

作者：

Guthrie, J. L. ^{[1
]}

Seah, C. ^{[1
]}

Brown, S. ^{[1
]}

Tang, P. ^{[1
]}

Jamieson, F. ^{[1
,2
]}

Drews, S. J. ^{[1
,2
,3
]}

机构：

[1] Minist Hlth & Long Term Care, Ontario Publ Hlth Labs, Toronto, ON M9P 3T1, Canada

[2] Univ Toronto, Dept Lab Med & Pathobiol, Toronto, ON, Canada

[3] Mt Sinai Hosp, Toronto, ON M5G 1X5, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 11期

关键词：

D O I：

10.1128/JCM.01551-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C-T) values of >35. IS481 PCRs with C-T values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

引用

页码：3798 / 3799

页数：2

共 50 条

[1] Real-time PCR assay targeting IS481 of Bordetella pertussis and molecular basis for detecting Bordetella holmesii
Reischl, U
Lehn, N
Sanden, GN
Loeffelholz, MJ
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (05) : 1963 - 1966
[2] A specific real-time PCR assay for the detection of Bordetella pertussis
Vincart, Benoit
De Mendonca, Ricardo
Rottiers, Sylvianne
Vermeulen, Francoise
Struelens, Marc J.
Denis, Olivier
JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 56 (07) : 918 - 920
[3] Performance of Bordetella pertussis IS481 real-time PCR in a vaccine trial setting
Gullsby, Karolina
Hallander, Hans O.
Bondeson, Kare
APMIS, 2007, 115 (12) : 1370 - 1375
[4] Multicenter Clinical Evaluation of a Real-Time PCR Assay for Bordetella pertussis
Uphofft, T. S.
Bard, J. Dien
Denys, G.
Oethinger, M.
Sinclair, W.
Young, S.
JOURNAL OF MOLECULAR DIAGNOSTICS, 2017, 19 (06): : 986 - 986
[5] Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis
Kolodkina, Valentina
Martinov, Vladimir
Babenko, Andrey
IRANIAN JOURNAL OF MICROBIOLOGY, 2014, 6 (03) : 140 - 148
[6] Triplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis
Xu, Yinghua
Xu, Yunqiang
Hou, Qiming
Yang, Ruifu
Zhang, Shumin
APMIS, 2010, 118 (09) : 685 - 691
[7] Further evidence that the IS481 target is suitable for real-time PCR detection of Bordetella pertussis
Cox, Hannah C.
Jacob, Kevin
Whiley, David M.
Bletchly, Cheryl
Nimmo, Graeme R.
Nissen, Michael D.
Sloots, Theo P.
PATHOLOGY, 2013, 45 (02) : 202 - 203
[8] Development of a real-time PCR for the identification of Bordetella pertussis and Bordetella parapertussis
Menard, A.
Lehours, P.
Sarlangue, J.
Bebear, C.
Megraud, F.
de Barbeyrac, B.
CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (04) : 419 - 423
[9] Addition of a homologous internal control to a real-time PCR assay for detection of Bordetella pertussis
Koidl, C
Bozic, M
Berg, J
Stöcher, M
Mühlbauer, G
Marth, E
Kessler, HH
CLINICAL CHEMISTRY, 2005, 51 (12) : 2404 - 2406
[10] Development of a multiplex real-time PCR assay for the detection of Bordetella pertussis and Bordetella parapertussis in a single tube reaction
Arbefeville, Sophie
Levi, Michael H.
Ferrieri, Patricia
JOURNAL OF MICROBIOLOGICAL METHODS, 2014, 97 : 15 - 19

← 1 2 3 4 5 →