The C-terminal region of OsWRKY30 is sufficient to confer enhanced resistance to pathogen and activate the expression of defense-related genes

被引：10

作者：

Lee, Heyoung ^{[1
]}

Ko, Yu Jin ^{[1
]}

Cha, Joo-Young ^{[1
]}

Park, Sang Ryeol ^{[1
]}

Ahn, Ilpyung ^{[1
]}

Hwang, Duk-Ju ^{[1
]}

机构：

[1] Natl Acad Agr Sci, Rural Dev Adm, Suwon 440707, South Korea

来源：

PLANT BIOTECHNOLOGY REPORTS | 2013年 / 7卷 / 03期

关键词：

Transcription factor; OsWRKY30; Defense signaling; WRKY70 TRANSCRIPTION FACTOR; CAMPESTRIS PV. CAMPESTRIS; RICE ORYZA-SATIVA; PLANT DEFENSE; ARABIDOPSIS-THALIANA; XANTHOMONAS-CAMPESTRIS; SIGNALING PATHWAYS; FULL VIRULENCE; DNA-BINDING; NEGATIVE REGULATORS;

D O I：

10.1007/s11816-012-0252-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

WRKY proteins are known to play major roles in defense signaling. We identified a pathogen-inducible and SA-inducible OsWRKY30. Our cDNA clone encodes the C-terminal region (CTR) of OsWRKY30. CTR-OsWRKY30 includes the C-terminal WRKY domain and nuclear localization sequence. CTR-OsWRKY30 was sufficient to bind W-box sequences (TTGACC/T). Over-expression of the CTR-OsWRKY30 resulted in enhanced resistance to pathogens in Arabidopsis and rice. Defense-related genes were constitutively expressed in transgenic Arabidopsis and rice over-expressing CTR-OsWRKY30. Based on promoter transient assays, CTR-OsWRKY30 is sufficient to activate OsPR10a promoter as much as full length OsWRKY30. Taken together, CTR-OsWRKY30 positively regulates defense signaling, thereby resulting in enhanced resistance to pathogens.

引用

页码：221 / 230

页数：10

共 2 条

[1] The C-terminal region of OsWRKY30 is sufficient to confer enhanced resistance to pathogen and activate the expression of defense-related genes
Heyoung Lee
Yu Jin Ko
Joo-Young Cha
Sang Ryeol Park
Ilpyung Ahn
Duk-Ju Hwang
Plant Biotechnology Reports, 2013, 7 : 221 - 230
[2] Enhanced Botrytis cinerea Resistance of Arabidopsis Plants Grown in Compost May Be Explained by Increased Expression of Defense-Related Genes, as Revealed by Microarray Analysis
Segarra, Guillem
Santpere, Gabriel
Elena, Georgina
Trillas, Isabel
PLOS ONE, 2013, 8 (02):

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