ANTIBACTERIAL ACTIVITY AND IN-VITRO CYTOTOXICITY ASSAY AGAINST BRINE SHRIMP USING SILVER NANOPARTICLES SYNTHESIZED FROM SARGASSUM ILICIFOLIUM

The increasing commercial applications of nanoparticles have wide pertinent in the field of medicine. Biological synthesis of Silver nanoparticles (AgNPs) are effective and environment friendly than chemical methods. In the present study, we demonstrated extracellular synthesis of AgNPs from Sargassum ilicifolium rapidly. The UV-Vis spectra of aqueous medium containing Ag+ ions showed peak at 414 nm corresponding to the plasmon resonance of AgNPs. SEM and TEM analysis showed formation of well-dispersed AgNPs in the range of 33-40 nm. Antibacterial activity against five clinical pathogens at various concentrations shows impediment in growth at various nanomolar concentrations. Further, the toxicity of biosynthesized AgNPs was tested against Artemia salina to evaluate the cytotoxic effect that displayed LD50 value of 10nM/ml.