A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue

被引：27
|
作者：
Xia, Xiaohu [1 ]
Xu, Ye [1 ]
Ke, Rongqin [1 ]
Zhang, Heng [2 ]
Zou, Mingqiang [3 ]
Yang, Wei [1 ]
Li, Qingge [1 ]
机构：
[1] Xiamen Univ, Sch Life Sci, Dept Biomed Sci, Engn Res Ctr Mol Diagnost,Minist Educ, Xiamen 361005, Fujian, Peoples R China
[2] Shenzhen Entry Exit Inspect & Quarantine Bur, Shenzhen 518067, Peoples R China
[3] Chinese Acad Inspect & Quarantine, Beijing 100123, Peoples R China
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 23期
基金：
中国国家自然科学基金;
关键词：
Detection; Chloramphenicol; Lateral flow immunoassay; Europium chelate; Nanoparticle; LINKED-IMMUNOSORBENT-ASSAY; SILICA NANOPARTICLES; CHICKEN MUSCLE; MILK; 2002/657/EC; VALIDATION; TISSUES; SHRIMP; HONEY; ELISA;
D O I：
10.1007/s00216-013-7210-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is similar to 100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.
引用
收藏
页码：7541 / 7544
页数：4
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