Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease

被引：801

作者：

Gratz, Scott J. ^{[1
]}

Cummings, Alexander M. ^{[2
]}

Nguyen, Jennifer N. ^{[3
]}

Hamm, Danielle C. ^{[4
]}

Donohue, Laura K. ^{[2
]}

Harrison, Melissa M. ^{[4
,6
]}

Wildonger, Jill ^{[3
]}

O'Connor-Giles, Kate M. ^{[1
,2
,5
]}

机构：

[1] Univ Wisconsin, Genet Training Program, Madison, WI 53706 USA

[2] Univ Wisconsin, Genet Lab, Madison, WI 53706 USA

[3] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

[4] Univ Wisconsin, Integrated Program Biochem, Madison, WI 53706 USA

[5] Univ Wisconsin, Lab Cell & Mol Biol, Madison, WI 53706 USA

[6] Univ Wisconsin, Sch Med & Publ Hlth, Dept Biomol Chem, Madison, WI 53706 USA

来源：

GENETICS | 2013年 / 194卷 / 04期

基金：

美国国家卫生研究院;

关键词：

ZINC-FINGER NUCLEASES; INDUCED GAP REPAIR; HOMOLOGOUS RECOMBINATION; GENE; EFFICIENT; MELANOGASTER; MUTAGENESIS; ENDONUCLEASE; INTERFERENCE; REPLACEMENT;

D O I：

10.1534/genetics.113.152710

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.

引用

页码：1029 / +

页数：17

共 31 条

[1] OLIGONUCLEOTIDE-DIRECTED SITE-SPECIFIC MUTAGENESIS IN DROSOPHILA-MELANOGASTER [J].

BANGA, SS ;

BOYD, JB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1735-1739

[2] CRISPR provides acquired resistance against viruses in prokaryotes [J].

Barrangou, Rodolphe ;

Fremaux, Christophe ;

Deveau, Helene ;

Richards, Melissa ;

Boyaval, Patrick ;

Moineau, Sylvain ;

Romero, Dennis A. ;

Horvath, Philippe .

SCIENCE, 2007, 315 (5819) :1709-1712

[3] Donor DNA Utilization During Gene Targeting with Zinc-Finger Nucleases [J].

Beumer, Kelly J. ;

Trautman, Jonathan K. ;

Mukherjee, Kusumika ;

Carroll, Dana .

G3-GENES GENOMES GENETICS, 2013, 3 (04) :657-664

[4] Efficient gene targeting in Drosophila by direct embryo injection with zinc-finger nucleases [J].

Beumer, Kelly J. ;

Trautman, Jonathan K. ;

Bozas, Ana ;

Liu, Ji-Long ;

Rutter, Jared ;

Gall, Joseph G. ;

Carroll, Dana .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (50) :19821-19826

[5]

Bibikova M, 2002, GENETICS, V161, P1169

[6] Genetic Analysis of Zinc-Finger Nuclease-Induced Gene Targeting in Drosophila [J].

Bozas, Ana ;

Beumer, Kelly J. ;

Trautman, Jonathan K. ;

Carroll, Dana .

GENETICS, 2009, 182 (03) :641-651

[7] Genome editing with RNA-guided Cas9 nuclease in Zebrafish embryos [J].

Chang, Nannan ;

Sun, Changhong ;

Gao, Lu ;

Zhu, Dan ;

Xu, Xiufei ;

Zhu, Xiaojun ;

Xiong, Jing-Wei ;

Xi, Jianzhong Jeff .

CELL RESEARCH, 2013, 23 (04) :465-472

[8] Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease [J].

Cho, Seung Woo ;

Kim, Sojung ;

Kim, Jong Min ;

Kim, Jin-Soo .

NATURE BIOTECHNOLOGY, 2013, 31 (03) :230-232

[9] Multiplex Genome Engineering Using CRISPR/Cas Systems [J].

Cong, Le ;

Ran, F. Ann ;

Cox, David ;

Lin, Shuailiang ;

Barretto, Robert ;

Habib, Naomi ;

Hsu, Patrick D. ;

Wu, Xuebing ;

Jiang, Wenyan ;

Marraffini, Luciano A. ;

Zhang, Feng .

SCIENCE, 2013, 339 (6121) :819-823

[10] Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems [J].

DiCarlo, James E. ;

Norville, Julie E. ;

Mali, Prashant ;

Rios, Xavier ;

Aach, John ;

Church, George M. .

NUCLEIC ACIDS RESEARCH, 2013, 41 (07) :4336-4343

← 1 2 3 4 →