Novel primers reveal wider diversity among marine aerobic anoxygenic phototrophs

被引:74
|
作者
Yutin, N
Suzuki, MT
Béjà, O [1 ]
机构
[1] Technion Israel Inst Technol, Dept Biol, IL-32000 Haifa, Israel
[2] Univ Maryland, Ctr Environm Sci, Chesapeake Biol Lab, Solomons, MD 20688 USA
关键词
D O I
10.1128/AEM.71.12.8958-8962.2005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aerobic anoxygenic phototrophic bacteria (AAnPs) were previously proposed to account for up to 11% of marine bacterioplankton and to potentially have great ecological importance in the world's oceans. Our data show that previously used primers based on the M subunit of anoxygenic photosynthetic reaction center genes (pufM) do not comprehensively identify the diversity of AAnPs in the ocean. We have designed and tested a new set of pufM-specific primers and revealed several new AAnP variants in environmental DNA samples and genomic libraries.
引用
收藏
页码:8958 / 8962
页数:5
相关论文
共 20 条
  • [1] Unsuspected diversity among marine aerobic anoxygenic phototrophs
    Béjà, O
    Suzuki, MT
    Heidelberg, JF
    Nelson, WC
    Preston, CM
    Hamada, T
    Eisen, JA
    Fraser, CM
    DeLong, EF
    NATURE, 2002, 415 (6872) : 630 - 633
  • [2] Unsuspected diversity among marine aerobic anoxygenic phototrophs
    Oded Béjà
    Marcelino T. Suzuki
    John F. Heidelberg
    William C. Nelson
    Christina M. Preston
    Tohru Hamada
    Jonathan A. Eisen
    Claire M. Fraser
    Edward F. DeLong
    Nature, 2002, 415 : 630 - 633
  • [3] A Metagenomic and Amplicon Sequencing Combined Approach Reveals the Best Primers to Study Marine Aerobic Anoxygenic Phototrophs
    Carlota R. Gazulla
    Ana María Cabello
    Pablo Sánchez
    Josep M. Gasol
    Olga Sánchez
    Isabel Ferrera
    Microbial Ecology, 2023, 86 : 2161 - 2172
  • [4] A Metagenomic and Amplicon Sequencing Combined Approach Reveals the Best Primers to Study Marine Aerobic Anoxygenic Phototrophs
    Gazulla, Carlota R.
    Cabello, Ana Maria
    Sanchez, Pablo
    Gasol, Josep M.
    Sanchez, Olga
    Ferrera, Isabel
    MICROBIAL ECOLOGY, 2023, 86 (03) : 2161 - 2172
  • [5] Seasonal changes and diversity of aerobic anoxygenic phototrophs in the Baltic Sea
    Masin, Michal
    Zdun, Agnieszka
    Ston-Egiert, Joanna
    Nausch, Monika
    Labrenz, Matthias
    Moulisova, Vladimira
    Koblizek, Michal
    AQUATIC MICROBIAL ECOLOGY, 2006, 45 (03) : 247 - 254
  • [6] Global diversity and distribution of aerobic anoxygenic phototrophs in the tropical and subtropical oceans
    Gazulla, Carlota R.
    Auladell, Adria
    Ruiz-Gonzalez, Clara
    Junger, Pedro C.
    Royo-Llonch, Marta
    Duarte, Carlos M.
    Gasol, Josep M.
    Sanchez, Olga
    Ferrera, Isabel
    ENVIRONMENTAL MICROBIOLOGY, 2022, 24 (05) : 2222 - 2238
  • [7] Novel halophilic aerobic anoxygenic phototrophs from a Canadian hypersaline spring system
    Julius T. Csotonyi
    Jolantha Swiderski
    Erko Stackebrandt
    Vladimir V. Yurkov
    Extremophiles, 2008, 12 : 529 - 539
  • [8] Novel halophilic aerobic anoxygenic phototrophs from a Canadian hypersaline spring system
    Csotonyi, Julius T.
    Swiderski, Jolantha
    Stackebrandt, Erko
    Yurkov, Vladimir V.
    EXTREMOPHILES, 2008, 12 (04) : 529 - 539
  • [9] New techniques reveal new mechanisms-The role of aerobic anoxygenic phototrophs in carbon cycling in the ocean
    Jiao, Nianzhi
    JOURNAL OF BIOTECHNOLOGY, 2008, 136 : S10 - S10
  • [10] Novel primers to identify a wider diversity of butyrate-producing bacteria
    Meng, Xianbin
    Shu, Qinglong
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2024, 40 (02):