Analysis of biofluids and chemical mixtures in non-deuterated solvents with H-1 diffusion-weighted PFG phase-sensitive double-quantum NMR spectroscopy

Two different types of one-and two-dimensional diffusion-weighted H-1 phase-sensitive pulsed field gradient double-quantum experiments (DWDQ) are presented and their application to a biological plasma sample is shown, The remarkable solvent suppression achieved with these pulse sequences and the high sensitivity of these experiments allows the determination of the diffusion coefficients of molecules at low concentration dissolved in non-deuterated solvents, Biofluids and complex chemical mixtures from combinatorial chemistry and from natural sources can be analysed with these experiments, and the different molecular species of the mixtures can be identified and assigned, based on their different diffusion coefficients, (C) 1997 John Wiley & Sons, Ltd.