A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living cells

被引：38
|
作者：
Gao, Congcong [1 ]
Zang, Shunping [1 ]
Nie, Longxue [1 ]
Tian, Yong [1 ]
Zhang, Rubo [1 ]
Jing, Jing [1 ]
Zhang, Xiaoling [1 ]
机构：
[1] Beijing Inst Technol, Beijing Key Lab Photoelect Electrophoton Convers, Key Lab Cluster Sci, Sch Chem,Minist Educ, Beijing 100081, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2019年 / 1066卷
基金：
中国国家自然科学基金;
关键词：
Ratiometric; Fluorescent probe; Alkaline phosphatase; SULFUR-DIOXIDE DERIVATIVES; COLORIMETRIC ASSAY; MECHANISM; STRATEGY;
D O I：
10.1016/j.aca.2019.03.043
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We report a sensitive fluorescence probe Ratio-ALP for in vivo ratiometric imaging of alkaline phosphatase (ALP). The probe was designed by conjugating a naphthalimide with a phosphate group as the responsive moiety for enzymatic reactions. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 0 to 200 U/L, its LOD is as low as 0.25 U/L. Ratio-ALP has good selectivity and sensitivity, high cell permeability, and low cytotoxicity. Furthermore, probe Ratio-ALP was also successfully applied to image endogenous ALP activity in living cells. (C) 2019 Elsevier B.V. All rights reserved.
引用
收藏
页码：131 / 135
页数：5
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