A new low-cost glucoamylase preparation for liquefaction and saccharification of starchy raw materials in a one-stage system was developed and characterized. A non-purified biocatalyst with a glucoamylase activity of 3.11 U/mg, an alpha-amylase activity of 0.12 WU/ing and a protein content of 0.04 mg protein/mg was obtained from a shaken-flask culture of the strain Aspergillus niger C-IV-4. Factors influencing the enzymatic hydrolysis of starchy materials such as reaction time, temperature and enzyme and substrate concentration were standardized to maximize the yield of glucose syrup. Thus, a 90% conversion of 5% starch, a 67.5% conversion of 5% potato flour and a 55% conversion of 5% wheat flour to sweet syrups containing up to 87% glucose was reached in 3 h using 1.24 glucoamylase U/mg hydrolyzed substrate. The application of such glucoamylase preparation and a commercially immobilized glucose isomerase for the production of glucose-fructose syrup in a two-stage system resulted in high production of stable glucose/fructose blends with a fructose content of 50%. A high concentration of fructose in obtained sweet syrups was achieved when isomerization was performed both in a batch and repeated batch process.
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Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
SK Innovat Co, 325 Exporo, Daejeon 305712, South KoreaKorea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
Seo, Young Bin
Park, Juyi
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Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South KoreaKorea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
Park, Juyi
Huh, In Young
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Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South KoreaKorea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
Huh, In Young
Hong, Soon-Kwang
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Myung Ji Univ, Div Biosci & Bioinformat, Yongin 449728, South KoreaKorea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea
Hong, Soon-Kwang
Chang, Yong Keun
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Korea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South KoreaKorea Adv Inst Sci & Technol, Dept Chem & Biomol Engn, Taejon 305701, South Korea