Isoglucose production from raw starchy materials based on a two-stage enzymatic system

被引:0
|
作者
Gromada, Anna [1 ]
Fiedurek, Jan [1 ]
Szczodrak, Janusz [1 ]
机构
[1] Marie Curie Sklodowska Univ, Dept Ind Microbiol, Inst Microbiol & Biotechnol, Fac Biol & Earth Sci, PL-20033 Lublin, Poland
关键词
Aspergillus niger; glucoamylase; glucose-fructose syrup; isomerization;
D O I
暂无
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A new low-cost glucoamylase preparation for liquefaction and saccharification of starchy raw materials in a one-stage system was developed and characterized. A non-purified biocatalyst with a glucoamylase activity of 3.11 U/mg, an alpha-amylase activity of 0.12 WU/ing and a protein content of 0.04 mg protein/mg was obtained from a shaken-flask culture of the strain Aspergillus niger C-IV-4. Factors influencing the enzymatic hydrolysis of starchy materials such as reaction time, temperature and enzyme and substrate concentration were standardized to maximize the yield of glucose syrup. Thus, a 90% conversion of 5% starch, a 67.5% conversion of 5% potato flour and a 55% conversion of 5% wheat flour to sweet syrups containing up to 87% glucose was reached in 3 h using 1.24 glucoamylase U/mg hydrolyzed substrate. The application of such glucoamylase preparation and a commercially immobilized glucose isomerase for the production of glucose-fructose syrup in a two-stage system resulted in high production of stable glucose/fructose blends with a fructose content of 50%. A high concentration of fructose in obtained sweet syrups was achieved when isomerization was performed both in a batch and repeated batch process.
引用
收藏
页码:141 / 148
页数:8
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