Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint

被引:52
|
作者
Fabritius, Marie [1 ]
Chtioui, Haithem [2 ]
Battistella, Giovanni [3 ]
Annoni, Jean-Marie [4 ]
Dao, Kim [2 ]
Favrat, Bernard [5 ]
Fornari, Eleonora [3 ,6 ]
Lauer, Estelle [7 ]
Maeder, Philippe [3 ]
Giroud, Christian [1 ]
机构
[1] CURML Univ Ctr Legal Med, UTCF Forens Toxicol & Chem Unit, CH-1011 Lausanne, Switzerland
[2] CHU Vaudois, Div Clin Pharmacol & Toxicol, CH-1011 Lausanne, Switzerland
[3] CHU Vaudois, Dept Radiol, CH-1011 Lausanne, Switzerland
[4] Univ Fribourg, Dept Med, Neurol Unit, CH-1700 Fribourg, Switzerland
[5] CURML Univ Ctr Legal Med, UMPT Unit Psychol & Traff Med, CH-1011 Lausanne, Switzerland
[6] Ctr Hosp Univ Vaudois Unit, CIBM Ctr Imagerie BioMed, CH-1015 Lausanne, Switzerland
[7] CURML Univ Ctr Legal Med, UTCF Forens Toxicol & Chem Unit, CH-1211 Geneva, Switzerland
基金
瑞士国家科学基金会;
关键词
Cannabis; Pharmacokinetic; Oral fluid; Whole blood; Heavy use; Occasional use; TANDEM MASS-SPECTROMETRY; PHARMACOKINETIC PROPERTIES; DELTA(9)-TETRAHYDROCANNABINOL THC; IMPAIRED DRIVERS; PLASMA; USERS; URINE; DRUGS; HEAVY; SERUM;
D O I
10.1007/s00216-013-7412-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A cross-over controlled administration study of smoked cannabis was carried out on occasional and heavy smokers. The participants smoked a joint (11 % Delta 9-tetrahydrocannabinol (THC)) or a matching placebo on two different occasions. Whole blood (WB) and oral fluid (OF) samples were collected before and up to 3.5 h after smoking the joints. Pharmacokinetic analyses were obtained from these data. Questionnaires assessing the subjective effects were administered to the subjects during each session before and after the smoking time period. THC, 11-hydroxy-THC (11-OH-THC) and 11-nor-9-carboxy-THC (THCCOOH) were analyzed in the blood by gas chromatography or liquid chromatography (LC)-tandem mass spectrometry (MS/MS). The determination of THC, THCCOOH, cannabinol (CBN), and Delta 9-tetrahydrocannabinolic acid A (THC-A) was carried out on OF only using LC-MS/MS. In line with the widely accepted assumption that cannabis smoking results in a strong contamination of the oral cavity, we found that THC, and also THC-A, shows a sharp, high concentration peak just after smoking, with a rapid decrease in these levels within 3 h. No obvious differences were found between both groups concerning THC median maximum concentrations measured either in blood or in OF; these levels were equal to 1,338 and 1,041 mu g/L in OF and to 82 and 94 mu g/L in WB for occasional and heavy smokers, respectively. The initial WB THCCOOH concentration was much higher in regular smokers than in occasional users. Compared with the occasional smokers, the sensation of confusion felt by the regular smokers was much less while the feeling of intoxication remained almost unchanged.
引用
收藏
页码:9791 / 9803
页数:13
相关论文
共 37 条
  • [1] Comparison of cannabinoid concentrations in oral fluid and whole blood between occasional and regular cannabis smokers prior to and after smoking a cannabis joint
    Marie Fabritius
    Haithem Chtioui
    Giovanni Battistella
    Jean-Marie Annoni
    Kim Dao
    Bernard Favrat
    Eleonora Fornari
    Estelle Lauer
    Philippe Maeder
    Christian Giroud
    Analytical and Bioanalytical Chemistry, 2013, 405 : 9791 - 9803
  • [2] Comparison of Cannabinoid Concentrations in Plasma, Oral Fluid and Urine in Occasional Cannabis Smokers After Smoking Cannabis Cigarette
    Marsot, A.
    Audebert, C.
    Attolini, L.
    Lacarelle, B.
    Micallef, J.
    Blin, O.
    JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES, 2016, 19 (03): : 411 - 422
  • [3] Cannabinoid disposition in oral fluid after controlled cannabis smoking in frequent and occasional smokers
    Newmeyer, Matthew N.
    Desrosiers, Nathalie A.
    Lee, Dayong
    Mendu, Damodara R.
    Barnes, Allan J.
    Gorelick, David A.
    Huestis, Marilyn A.
    DRUG TESTING AND ANALYSIS, 2014, 6 (10) : 1002 - 1010
  • [4] Blood and Oral Fluid Cannabinoid Profiles of Frequent and Occasional Cannabis Smokers
    Hoffman, Melissa A.
    Hubbard, Jacqueline A.
    Sobolesky, Philip M.
    Smith, Breland E.
    Suhandynata, Raymond T.
    Sanford, Sandra
    Sones, Emily G.
    Ellis, Shannon
    Umlauf, Anya
    Huestis, Marilyn A.
    Grelotti, David J.
    Grant, Igor
    Marcotte, Thomas D.
    Fitzgerald, Robert L.
    JOURNAL OF ANALYTICAL TOXICOLOGY, 2021, 45 (08) : 851 - 862
  • [5] Oral fluid cannabinoid concentrations following controlled smoked cannabis in chronic frequent and occasional smokers
    Sebastien Anizan
    Garry Milman
    Nathalie Desrosiers
    Allan J. Barnes
    David A. Gorelick
    Marilyn A. Huestis
    Analytical and Bioanalytical Chemistry, 2013, 405 : 8451 - 8461
  • [6] Oral fluid cannabinoid concentrations following controlled smoked cannabis in chronic frequent and occasional smokers
    Anizan, Sebastien
    Milman, Garry
    Desrosiers, Nathalie
    Barnes, Allan J.
    Gorelick, David A.
    Huestis, Marilyn A.
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2013, 405 (26) : 8451 - 8461
  • [7] Cannabinoid concentrations in confiscated cannabis samples and in whole blood and urine after smoking CBD-rich cannabis as a “tobacco substitute”
    Marianne Hädener
    Tim J. Gelmi
    Marie Martin-Fabritius
    Wolfgang Weinmann
    Matthias Pfäffli
    International Journal of Legal Medicine, 2019, 133 : 821 - 832
  • [8] Cannabinoid concentrations in confiscated cannabis samples and in whole blood and urine after smoking CBD-rich cannabis as a "tobacco substitute"
    Hadener, Marianne
    Gelmi, Tim J.
    Martin-Fabritius, Marie
    Weinmann, Wolfgang
    Pfaffli, Matthias
    INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2019, 133 (03) : 821 - 832
  • [9] Cannabinoid Stability in Authentic Oral Fluid after Controlled Cannabis Smoking
    Lee, Dayong
    Milman, Garry
    Schwope, David M.
    Barnes, Allan J.
    Gorelick, David A.
    Huestis, Marilyn A.
    CLINICAL CHEMISTRY, 2012, 58 (07) : 1101 - 1109
  • [10] Oral fluid cannabinoids in chronic frequent cannabis smokers during ad libitum cannabis smoking
    Lee, Dayong
    Vandrey, Ryan
    Mendu, Damodara R.
    Murray, Jeannie A.
    Barnes, Allan J.
    Huestis, Marilyn A.
    DRUG TESTING AND ANALYSIS, 2015, 7 (06) : 494 - 501