The goals of the study were to analyze differences as a function of gender, age, and educational level in sexism and alexithymia in a nonclinical and in a clinical sample, and to explore the relation between these constructs. A descriptive and correlational cross-sectional methodology was used. The Ambivalent Sexism Inventory (Glick & Fiske, 1996) and the Toronto Alexithymia Scale (Parker et al. 1993) were administered. The sample comprised 989 participants from the Basque Country, aged between 18 and 65 years. The results revealed: 1) Significantly higher scores in the males in sexism (hostile, benevolent, and ambivalent) and in alexithymia (difficulties to express emotions and external-oriented thinking) in both samples; in the total alexithymia score, the males had significantly higher scores only in the nonclinical sample; 2) As of 55 years of age, a significant increase in benevolent and ambivalent sexism, and in difficulties to identify emotions, external-oriented thinking, and in the total alexithymia score were observed (only in the nonclinical sample); however, no changes with age were observed in hostile sexism and in difficulties to express emotions; 3) A decrease in sexism and alexithymia as the educational level increased; and 4) Significant positive correlations between sexism and alexithymia.