Genome sizes of the entomopathogenic nematodes Steinernema carpocapsae and Heterorhabditis bacteriophora (Nematoda: Rhabditida)

被引:8
|
作者
Grenier, E
Catzeflis, FM
Abad, P
机构
[1] INRA,LAB BIOL INVERTEBRES,F-06606 ANTIBES,FRANCE
[2] UNIV MONTPELLIER 2,LAB PALEONTOL,ISEM,UMR 5554 CNRS,F-34095 MONTPELLIER 5,FRANCE
关键词
Steinernema carpocapsae; Heterorhabditis bacteriophora; DNA complexity; C(0)t curves;
D O I
10.1017/S0031182096008736
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
There is currently no information on the genome size and complexity in the entomoparasitic nematodes Steinernema and Heterorhabditis. DNA reassociation kinetics were used to determine the genome size and complexity in 2 species: Steinernema carpocapsae and Heterorhabditis bacteriophora. C(0)t curves derived from renaturation kinetics of denatured DNA indicate that the genomes of these entomoparasitic rhabditids are different both in size and complexity. Genome sizes were estimated at 2.3 x 10(8) bp for S. carpocapsae and 3.9 x 10(7) bp for H. bacteriophora and repetitive DNA contents were found to represent 39% and 51% of these respective genomes.
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页码:497 / 501
页数:5
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