1α,25-Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow-derived macrophages

被引:3
|
作者
Wang, Dong [1 ,2 ]
Gu, Jian-Hong [1 ,2 ]
Chen, Yang [1 ,2 ]
Zhao, Hong-Yan [1 ,2 ]
Liu, Wei [1 ,2 ]
Song, Rui-Long [1 ,2 ]
Bian, Jian-Chun [1 ,2 ]
Liu, Xue-Zhong [1 ,2 ]
Yuan, Yan [1 ,2 ]
Liu, Zong-Ping [1 ,2 ]
机构
[1] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China
[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
基金
美国国家科学基金会; 中国国家自然科学基金;
关键词
1; alpha; 25-dihydroxyvitamin D-3; rat bone marrow mononuclear cells; osteoclast; bone absorption; VITAMIN-D; C-FMS; EXPRESSION; RECEPTOR; PROTEIN; CELLS;
D O I
10.3892/etm.2015.2632
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The steroid hormone 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25-(OH) 2D3] plays an important role in maintaining a balance in calcium and bone metabolism. To study the effects of 1 alpha, 25-(OH)(2)D-3 on osteoclast (OC) formation and bone resorption, OC differentiation was induced in bone marrow-derived mononuclear cells from Wistar rats with the addition of macrophage colony stimulating factor and receptor activator for nuclear factor-.B ligand in vitro. Cells were then treated with 1 alpha, 25-(OH)(2)D-3 at 10-9, 10-8 or 10-7 mol/l. OCs were identified using tartrate-resistant acid phosphatase staining and activity was monitored in the absorption lacunae by scanning electron microscopy. Expression levels of functional proteins associated with bone absorption, namely carbonic anhydrase II, cathepsin K and matrix metalloproteinase-9 were evaluated by western blot analysis. The results showed that 1 alpha,25-(OH)(2)D-3 inhibited the formation and activation of OCs in a dose-dependent manner and downregulated the expression levels of bone absorption-associated proteins.
引用
收藏
页码:1039 / 1044
页数:6
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