Enzymatic-catalyzed synthesis of alkylglycosides in monophasic and biphasic systems. II. The reverse hydrolysis reaction

被引：21

作者：

Ismail, A ^{[1
]}

Soultani, S ^{[1
]}

Ghoul, M ^{[1
]}

机构：

[1] ENSAIA, CNRS, Lab Sci Genie Chim, F-54505 Vandoeuvre Nancy, France

来源：

JOURNAL OF BIOTECHNOLOGY | 1999年 / 69卷 / 2-3期

关键词：

alkylglycosides; beta-glucosidase; reverse hydrolysis; equilibrium shift; system state;

D O I：

10.1016/S0168-1656(99)00042-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The beta-glucosidase catalyzed synthesis of butylglucoside through the condensation of glucose and butanol was carried out in monophasic and biphasic systems. Water was indispensable for the enzyme activity. In the case of the monophasic system, product concentration increased with the increase of water content, but at high levels of water the equilibrium shifted towards hydrolysis and therefore the butylglucoside concentration decreased. An optimal water content of about 8-13% (v/v) was found. However, in the case of biphasic system, the increase of the aqueous/butanol phase ratio (v/v) was accompanied by a decrease of the butylglucoside concentration. An analysis of the causes of the equilibrium shift is given. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：145 / 149

页数：5

共 28 条

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JOURNAL OF BIOTECHNOLOGY, 1999, 69 (2-3) : 135 - 143
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