Antifungal activity of gallic acid purified from Terminalia nigrovenulosa bark against Fusarium solani

被引:75
|
作者
Dang-Minh-Chanh Nguyen [1 ,2 ]
Seo, Dong-Jun [1 ]
Lee, Hyang-Burm [1 ]
Kim, In-Seon [1 ]
Kim, Kil-Yong [1 ]
Park, Ro-Dong [1 ]
Jung, Woo-Jin [1 ]
机构
[1] Chonnam Natl Univ, Div Appl Biosci & Biotechnol, IEFA, Coll Agr & Life Sci, Kwangju 500757, South Korea
[2] Western Highlands Agr Forestry Sci Inst, Buon Ma Thuot City, Vietnam
关键词
Terminalia nigrovenulosa; Gallic acid; Biocontrol; Fusarium solani; GROWTH-PROMOTING RHIZOBACTERIA; SYSTEMIC RESISTANCE; ANTIMICROBIAL ACTIVITIES; BIOLOGICAL-CONTROL; ESSENTIAL OILS; PLANT; EXTRACTS; INDUCTION; CUCUMBER; DISEASE;
D O I
10.1016/j.micpath.2013.01.001
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The antifungal activities of methanolic extracts from Terminalia nigrovenulosa bark (TNB) was investigated for effects on the initial growth of mycelia against Fusarium solani. The ethyl acetate fraction separated from TNB demonstrated the highest antifungal activity against F. solani. The antifungal compound was isolated from TNB using silica gel column and Sephadex LH-20 chromatography combined with thin-layer chromatography and high performance liquid chromatography. Structural identification of the antifungal compound was conducted using H-1 NMR, C-13 NMR, and liquid chromatography-tandem mass spectrometry. The purified antifungal compound was gallic acid (GA) or 3,4,5-trihydroxy benzoic acid. Purified-GA possesses the high antifungal activity against E solani, and that antifungal activity was dosage-dependent. The hyphae became collapsed and shrunken after 24 h incubation with GA (500 ppm). In pot experiments, the application of TNB crude extract was found to be effective in controlling the cucumber Fusarium root rot disease by enhancing activities of chitinase, peroxidase thereby promoting the growth of plants. The applied TNB extract significantly suppressed root rot disease compared to control. It resulted in 33, 75 and 81% disease suppression with 100, 500 and 1000 ppm of TNB crude extract, respectively. The study effectively demonstrated biological activities of the TNB extract, therefore suggesting the application of TNB for the control of soil-borne diseases of cucumber plants. (C) 2013 Elsevier Ltd. All rights reserved.
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页码:8 / 15
页数:8
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