Chondrogenic differentiation of mouse embryonic stem cells promoted by mature chondrocytes

被引：7
|
作者：
Xie Feng [1 ]
Zhang WenJie [1 ]
Chen FanFan [1 ]
Zhou GuangDong [1 ]
Cui Lei [1 ]
Liu Wei [1 ]
Cao Yilin [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Natl Tissue Engn Ctr China, Shanghai Peoples Hosp 9,Dept Plast & Reconstruct, Shanghai 200011, Peoples R China
来源：
SCIENCE IN CHINA SERIES C-LIFE SCIENCES | 2008年 / 51卷 / 09期
基金：
中国国家自然科学基金;
关键词：
chondrocytes; co-culture; differentiation; embryonic stem cells;
D O I：
10.1007/s11427-008-0111-z
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
In order to direct embryonic stem (ES) cells to differentiate into chondrocytes, a chondrogenic environment provided by mature chondrocytes was investigated. Flk-1 positive cells sorted from pre-differentiated mouse ES cells were mixed with adult porcine articular chondrocytes, seeded on biodegradable scaffolds, and then implanted subcutaneously into nude mice. The cell-scaffold complexes formed cartilage tissues after 4 weeks, which was demonstrated by histology and anti-type II collagen antibody staining. Positive staining of mouse Major Histocompatibility Complex class I molecules confirmed that part of the chondrocytes were derived from mouse ES cells. The current study established a new approach for directing ES cell differentiation.
引用
收藏
页码：774 / 778
页数：5
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