D-lactic acid production from Xylose in engineered Escherichia coli SZ470

被引：0

作者：

Zheng, Zhaomin ^{[1
]}

Tian, Tian ^{[1
]}

Wang, Jinhua ^{[1
]}

Wang, Yongze ^{[1
]}

Zhou, Shengde ^{[1
]}

机构：

[1] Hubei Univ Technol, Coll Bioengn, Minist Educ, Key Lab Fermentat Engn, Wuhan 430068, Peoples R China

来源：

BIOTECHNOLOGY, CHEMICAL AND MATERIALS ENGINEERING II, PTS 1 AND 2 | 2013年 / 641-642卷

关键词：

Lactic acid fermentation; Escherichia coli; xylose; polylactic acid(PLA);

D O I：

10.4028/www.scientific.net/AMR.641-642.721

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

WD100, knocked out adhE of Escherichia coli SZ470 and inserted ldhA into Escherichia coli WD01, was genetically engineered to utilize xylose. D-lactate production was investigated for shake flask cultures with xylose. In 64h WD 100 produce 10.1g/L D-lactate in the shaking flask And it consumed 25g/L xylose during the ending of fermentation. This volumetric productivity with xylose is 0.14 g.L-1.h(-1). Because of pyruvate decarboxylase (poxB) expressed in flask fermention,acetate production was up to 4.7g/L. Succinate, formate, ethanol was also produced as a minor product during fermentation.

引用

页码：721 / 724

页数：4

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