Horseradish peroxidase sol-gel immobilized for chemiluminescence measurements of alkaline-phosphatase activity

被引：42
|
作者：
Díaz, AN [1 ]
Sánchez, FG [1 ]
Ramos, MC [1 ]
Torijas, MC [1 ]
机构：
[1] Univ Malaga, Fac Ciencias, Dept Quim Analit, E-29071 Malaga, Spain
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2002年 / 82卷 / 2-3期
关键词：
alkaline-phosphatase HRP; enhanced chemiluminescence; biosensor; 2-naphthyl phosphate;
D O I：
10.1016/S0925-4005(01)01003-6
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
2-Naphthyl phosphate is a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction. Alkaline-phosphatase hydrolyses the phosphate group and produces 2-naphthol. This compound is an enhancer of the chemiluminescence (CL) reaction. The influence of 2-naphthyl phosphate and the incubation time on the chemiluminescence reaction. have been studied. The horseradish peroxidase (HRP) was immobilized in a sol-gel matrix, obtaining a biosensor for alkaline-phosphatase. The relative standard deviations using free HRP in solution and the immobilized one were 3.2 (n = 4) and 9.3% (n = 3). respectively. The detection limits for alkaline-phosphatase using free and immobilized HR-P were 128 and 106 muU/ml. respectively. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：176 / 179
页数：4
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