Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels

被引：10

作者：

Wei, Yueteng ^{[1
]}

Liu, Ru ^{[1
]}

Wang, Yaling ^{[1
]}

Zhao, Yuliang ^{[1
]}

Cai, Zhifang ^{[1
]}

Gao, Xueyun ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Biomed Effects Nanomat & Nanosaftey, Beijing 100049, Peoples R China

来源：

ANALYST | 2013年 / 138卷 / 08期

关键词：

NUCLEIC-ACIDS; DEOXYRIBONUCLEIC ACID; METAL IONS; DNA; LUMINESCENCE; COMPLEXES; SPECTROMETRY; NUCLEOTIDES; ENHANCEMENT; CADMIUM;

D O I：

10.1039/c3an36795a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A terbium based fluorescent probe was synthesized by coordinating terbium ions with a designed oligonucleotides (5'-ATATGGGGGATAT-3', termed GH5). GH5 improved the fluorescence of terbium ions by four orders of magnitude. The fluorescence enhancement of terbium ions by different oligonucleotides sequences indicated that the polyguanine loop of the hairpin GH5 is key to enhance terbium ion emission. The quantum yield of Tb-GH5 probe was 10.5% and the probe was photo-stable. The result of conductivity titration indicated that the stoichiometry of the probe is 3.5 Tb: 1 GH5, which is confirmed by fluorescence titration. This probe had high sensitivity and specificity for the detection of lead ions. The fluorescence intensity of this probe was linear with respect to lead concentration over a range 0.3-2.1 nM (R-2 = 0.99). The limit of detection for lead ions was 0.1 nM at a signal-to-noise ratio of 3.

引用

页码：2302 / 2307

页数：6