Isolation rates of Campylobacter fetus subsp venerealis from bovine preputial samples via passive filtration on nonselective medium versus selective medium, with and without transport medium

被引:18
|
作者
Chaban, Bonnie [1 ]
Guerra, Alvaro Garcia [2 ]
Hendrick, Steven H. [2 ]
Waldner, Cheryl L. [2 ]
Hill, Janet E. [1 ]
机构
[1] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Microbiol, Saskatoon, SK S7N 5B4, Canada
[2] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 5B4, Canada
关键词
ENRICHMENT MEDIUM; PCR ASSAY; BULLS; IDENTIFICATION; DIFFERENTIATION; INFECTIONS; SECRETIONS; DIAGNOSIS; CATTLE;
D O I
10.2460/ajvr.74.8.1066
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Objective-To compare the recovery rates of Campylobacter fetus subsp venerealis (Cfv) from preputial scrapings of infected bulls with passive filtration on selective medium versus nonselective medium, with and without transport medium. Samples-217 preputial scrapings from 12 bulls (4 naturally and 8 artificially infected with Cfv). Procedures-Preputial scrapings were collected in 2 mL of PBS solution and bacteriologically cultured directly on Skirrow medium or passively filtered through 0.65-mu m filters onto blood agar, with or without 24 hour preincubation in modified Weybridge transport enrichment medium (TEM). After 72 hours, plates were examined for Cfv and bacterial and fungal contamination or overgrowth. Results-Passive filtration of fresh preputial scrapings onto blood agar yielded significantly higher recovery rates of Cfv (86%) than direct plating on Skirrow medium (32%), whereas recovery from TEM was poor for both media (35% and 40%, respectively). Skirrow cultures without TEM were significantly more likely to have fungal contamination than were cultures performed with any other technique, and fungal contamination was virtually eliminated by passive filtration onto blood agar. Bacterial contamination by Pseudomonas spp was significantly more common with Skirrow medium versus passive filtration on blood agar, regardless of TEM use. Conclusions and Clinical Relevance-The use of transport medium and the choice of culture medium had significant effects on Cfv recovery and culture contamination rates from clinical samples. Both factors should be considered when animals are tested for this pathogen.
引用
收藏
页码:1066 / 1069
页数:4
相关论文
共 8 条
  • [1] Effect of transport enrichment medium, transport time, and growth medium on the detection of Campylobacter fetus subsp venerealis
    Monke, HJ
    Love, BC
    Wittum, TE
    Monke, DR
    Byrum, BA
    JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 2002, 14 (01) : 35 - 39
  • [2] ISOLATION OF VIBRIO FETUS FROM BOVINE PREPUTIAL FLUID, USING MILLIPORE FILTERS AND AN ANTIBIOTIC MEDIUM
    SHEPLER, VM
    PLUMER, GJ
    FABER, JE
    AMERICAN JOURNAL OF VETERINARY RESEARCH, 1963, 24 (101) : 749 - &
  • [3] MODIFIED SELECTIVE MEDIUM FOR ISOLATION OF CAMPYLOBACTER SPP FROM FECES - COMPARISON WITH PRESTON MEDIUM, A BLOOD-FREE MEDIUM, AND A FILTRATION SYSTEM
    GOOSSENS, H
    DEBOECK, M
    COIGNAU, H
    VLAES, L
    VANDENBORRE, C
    BUTZLER, JP
    JOURNAL OF CLINICAL MICROBIOLOGY, 1986, 24 (05) : 840 - 843
  • [4] ENRICHMENT MEDIUM AND CONTROL-SYSTEM FOR ISOLATION OF CAMPYLOBACTER-FETUS SUBSP JEJUNI FROM STOOLS
    CHAN, FTH
    MACKENZIE, AMR
    JOURNAL OF CLINICAL MICROBIOLOGY, 1982, 15 (01) : 12 - 15
  • [5] Effect of sample pooling and transport conditions on the clinical sensitivity of a real-time polymerase chain reaction assay for Campylobacter fetus subsp venerealis in preputial samples from bulls
    Garcia-Guerra, Alvaro
    Waldner, Cheryl L.
    Pellegrino, Andrea
    Macdonald, Nicole
    Chaban, Bonnie
    Hill, Janet E.
    Hendrick, Steven H.
    CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 2016, 80 (01): : 32 - 39
  • [6] Comparison of various culture methods (Skirrow medium, a blood-free medium and a filtration system enriched in Bolton and Preston broths) for isolation of Campylobacter spp. from raw meat samples
    Bi, Shui-lian
    Shi, Lei
    Yan, He
    Meng, He-cheng
    ANNALS OF MICROBIOLOGY, 2013, 63 (01) : 179 - 185
  • [7] Comparison of various culture methods (Skirrow medium, a blood-free medium and a filtration system enriched in Bolton and Preston broths) for isolation of Campylobacter spp. from raw meat samples
    Shui-lian Bi
    Lei Shi
    He Yan
    He-cheng Meng
    Annals of Microbiology, 2013, 63 : 179 - 185
  • [8] Head-to-head comparison of CAMPYAIR aerobic culture medium versus standard microaerophilic culture for Campylobacter isolation from clinical samples
    Levican, Arturo
    Varela, Carmen
    Porte, Lorena
    Weitzel, Thomas
    Briceno, Isabel
    Guerra, Francisco
    Mena, Benjamin
    Hinton Jr, Arthur
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2023, 13