Two versatile shuttle vectors for Thermus thermophilus-Escherichia coli containing multiple cloning sites, lacZα gene and kanamycin or hygromycin resistance marker

被引：10

作者：

Fujita, Atsushi ^{[1
]}

Misumi, Yoshio ^{[2
]}

Koyama, Yoshinori ^{[3
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, Tsukuba, Ibaraki 3058566, Japan

[2] Fukuoka Univ, Sch Med, Dept Cell Biol, Jonan Ku, Fukuoka 8140180, Japan

[3] Natl Inst Adv Ind Sci & Technol, Bioprod Res Inst, Tsukuba, Ibaraki 3058566, Japan

来源：

PLASMID | 2012年 / 67卷 / 03期

关键词：

Thermus thermophilus; Shuttle vector; Kanamycin; Hygromycin; EXTREME THERMOPHILE;

D O I：

10.1016/j.plasmid.2011.12.013

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two versatile shuttle vectors for Therm us thermophilus and Escherichia coli were developed on the basis of the T. thermophilus cryptic plasmid pTT8 and E. coli vector pUC13. These shuttle vectors, pTRK1T and pTRH1T, carry a gene encoding a protein homologous to replication protein derived from pTT8, a replicon for E. coli, new multiple cloning sites and a lacZ alpha gene from E. coli vector pUC13, and also have a gene encoding a thermostable protein that confers resistance to kanamycin or hygromycin, which can be used as a selection marker in T. thermophilus. These shuttle vectors are useful to develop enzymes and proteins of biotechnological interest. We also constructed a plasmid, pUC13T, which carries the same multiple cloning sites of pTRK1T and pTRH1T. These vectors should facilitate cloning procedures both in E. coli and T. thermophilus. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：272 / 275

页数：4

共 8 条

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GENE, 1982, 19 (02) : 211 - 219
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[6] Development of a new host–vector system for colour selection of cloned DNA inserts using a newly designed β-galactosidase gene containing multiple cloning sites in Thermus thermophilus HB27
Atsushi Fujita
Yoshio Misumi
Extremophiles, 2017, 21 : 1111 - 1117
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Fujita, Atsushi
Misumi, Yoshio
EXTREMOPHILES, 2017, 21 (06) : 1111 - 1117
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