Effete, a Drosophila Chromatin-Associated Ubiquitin-Conjugating Enzyme That Affects Telomeric and Heterochromatic Position Effect Variegation

被引:11
|
作者
Cipressa, Francesca [1 ,2 ]
Romano, Sabrina [4 ]
Centonze, Silvia [4 ]
zur Lage, Petra I. [5 ]
Verni, Fiammetta [2 ,3 ]
Dimitri, Patrizio [2 ,3 ]
Gatti, Maurizio [2 ,3 ]
Cenci, Giovanni [1 ,2 ]
机构
[1] Univ Aquila, Dipartimento Sci Clin Applicate & Biotecnol, I-67010 Laquila, Italy
[2] Univ Rome, Dipartimento Biol & Biotecnol C Darwin, I-00185 Rome, Italy
[3] Univ Rome, Ist Pasteur, Fdn Cenci Bolognetti, I-00185 Rome, Italy
[4] Univ Salento, Dipartimento Sci & Tecnol Biol & Ambientali, I-73100 Lecce, Italy
[5] Univ Edinburgh, Sch Biomed Sci, Edinburgh EH8 9XD, Midlothian, Scotland
来源
GENETICS | 2013年 / 195卷 / 01期
关键词
ELEMENT INSERTIONAL MUTAGENESIS; CHROMOSOME ENDS; DNA-DAMAGE; PROTEIN; GENE; MELANOGASTER; MAINTENANCE; COMPLEX; PROTECTION; ENCODES;
D O I
10.1534/genetics.113.153320
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Drosophila telomeres are elongated by the transposition of telomere-specific retrotransposons rather than telomerase activity. Proximal to the terminal transposon array, Drosophila chromosomes contain several kilobases of a complex satellite DNA termed telomere-associated sequences (TASs). Reporter genes inserted into or next to the TAS are silenced through a mechanism called telomere position effect (TPE). TPE is reminiscent of the position effect variegation (PEV) induced by Drosophila constitutive heterochromatin. However, most genes that modulate PEV have no effect on TPE, and systematic searches for TPE modifiers have so far identified only a few dominant suppressors. Surprisingly, only a few of the genes required to prevent telomere fusion have been tested for their effect on TPE. Here, we show that with the exception of the effete (eff; also called UbcD1) mutant alleles, none of the tested mutations at the other telomere fusion genes affects TPE. We also found that mutations in eff, which encodes a class I ubiquitin-conjugating enzyme, act as suppressors of PEV. Thus, eff is one of the rare genes that can modulate both TPE and PEV. Immunolocalization experiments showed that Eff is a major constituent of polytene chromosomes. Eff is enriched at several euchromatic bands and interbands, the TAS regions, and the chromocenter. Our results suggest that Eff associates with different types of chromatin affecting their abilities to regulate gene expression.
引用
收藏
页码:147 / +
页数:14
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  • [1] Effete, an E2 ubiquitin-conjugating enzyme with multiple roles in Drosophila development and chromatin organization
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  • [2] POSITION EFFECT VARIEGATION IN DROSOPHILA IS ASSOCIATED WITH AN ALTERED CHROMATIN STRUCTURE
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  • [3] Bonus, a Drosophila TIF1 homolog, is a chromatin-associated protein that acts as a modifier of position-effect variegation
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    Ner, SS
    Hales, KG
    Grigliatti, TA
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    Bellen, HJ
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  • [4] The dose of a putative ubiquitin-specific protease affects position-effect variegation in Drosophila melanogaster
    Henchoz, S
    DeRubertis, F
    Pauli, D
    Spierer, P
    MOLECULAR AND CELLULAR BIOLOGY, 1996, 16 (10) : 5717 - 5725
  • [5] Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification
    Roest, HP
    vanKlaveren, J
    deWit, J
    vanGurp, CG
    Koken, MHM
    Vermey, M
    vanRoijen, JH
    Hoogerbrugge, JW
    Vreeburg, JTM
    Baarends, WM
    Bootsma, D
    Grootegoed, JA
    Hoeijmakers, JHJ
    CELL, 1996, 86 (05) : 799 - 810
  • [6] pitkinD, a novel gain-of-function enhancer of position-effect variegation, affects chromatin regulation during oogenesis and early embryogenesis in drosophila
    Kuhfittig, S
    Szabad, J
    Schotta, G
    Hoffmann, J
    Máthé, E
    Reuter, G
    GENETICS, 2001, 157 (03) : 1227 - 1244