Quantitative Field Testing Heterodera glycines from Metagenomic DNA Samples Isolated Directly from Soil under Agronomic Production

被引：6
|
作者：
Li, Yan [1 ]
Lawrence, Gary W. [1 ]
Lu, Shien [1 ]
Balbalian, Clarissa [1 ]
Klink, Vincent P. [2 ]
机构：
[1] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA
[2] Mississippi State Univ, Dept Biol Sci, Mississippi State, MS 39762 USA
来源：
PLOS ONE | 2014年 / 9卷 / 02期
关键词：
UNITED-STATES; CAENORHABDITIS-ELEGANS; MAJOR MELOIDOGYNE; IDENTIFICATION; NEMATODES; QUANTIFICATION; EXPRESSION; JUVENILES; SOLANI; SCHEME;
D O I：
10.1371/journal.pone.0089887
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.
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页数：8
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