Two-liquid hanging-drop vapor-diffusion technique of protein crystallization

被引：5
|
作者：
Adachi, H
Matsumura, H
Takano, K
Niino, A
Inoue, T
Mori, Y
Sasaki, T
机构：
[1] Osaka Univ, Dept Elect Engn, Suita, Osaka 5650871, Japan
[2] Osaka Univ, SOSHO Project Crystal Design Project, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Venture Business Lab, Suita, Osaka 5650871, Japan
[4] Osaka Univ, Dept Chem Mat, Suita, Osaka 5650871, Japan
[5] Osaka Univ, Dept Mat & Life Sci, Suita, Osaka 5650871, Japan
[6] JST, PRESTO, Suita, Osaka 5650871, Japan
来源：
JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS | 2004年 / 43卷 / 1A-B期
关键词：
protein crystal growth; interface; hanging drop; vapor diffusion technique; two-liquid system;
D O I：
10.1143/JJAP.43.L79
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
A new method of protein crystallization, the two-liquid hanging-drop vapor-diffusion technique including the double-hanging-drop and the sandwiched-hanging-drop techniques, has been developed. This method uses a very dense insoluble liquid in a traditional hanging-drop technique. The double-hanging-drop vapor-diffusion technique enables us to remove the crystals easily without causing mechanical damage. In the sandwiched-hanging-drop technique, the vapor diffusion rate can be altered, which is a useful method for screening protein crystal growth conditions.
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页码：L79 / L81
页数：3
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