Improving the quality of electron tomography image volumes using pre-reconstruction filtering

被引:13
|
作者
Maiorca, Mauro [1 ,2 ]
Hanssen, Eric [2 ,3 ]
Kazmierczak, Edmund [4 ]
Maco, Bohumil [5 ]
Kudryashev, Misha [6 ]
Hall, Richard [7 ]
Quiney, Harry [2 ,8 ]
Tilley, Leann [1 ,2 ]
机构
[1] Univ Melbourne, Dept Biochem & Mol Biol, Mol Sci & Biotechnol Inst Bio21, Melbourne, Vic 3010, Australia
[2] Univ Melbourne, Ctr Excellence Coherent Xray Sci, Australian Res Council, Melbourne, Vic 3010, Australia
[3] Univ Melbourne, Mol Sci & Biotechnol Inst Bio21, Electron Microscopy Unit, Melbourne, Vic 3010, Australia
[4] Univ Melbourne, Dept Comp Sci & Software Engn, Melbourne, Vic 3010, Australia
[5] La Trobe Univ, Dept Biochem, Bundoora, Vic 3086, Australia
[6] Univ Basel, Biozentrum, Ctr Cellular Imaging & NanoAnalyt C CINA, CH-4003 Basel, Switzerland
[7] Univ Melbourne, Melbourne Univ Virtual Environm Simulat, Melbourne, Vic 3010, Australia
[8] Univ Melbourne, Sch Phys, Melbourne, Vic 3010, Australia
基金
澳大利亚研究理事会; 英国医学研究理事会;
关键词
Electron tomography; Denoising; Plasmodium; Non-linear anisotropic diffusion filtering; CRYOELECTRON TOMOGRAPHY; NOISE-REDUCTION; SCALE-SPACE; BIOLOGICAL SPECIMENS; NONLINEAR DIFFUSION; MICROSCOPY; SEGMENTATION; CELLS; 3D; PERSPECTIVES;
D O I
10.1016/j.jsb.2012.05.019
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Electron tomography produces highly magnified 3D image volumes useful for investigating the structure and function of cellular components. Image quality is degraded by multiple scattering events and quantum noise, which depend on the angle at which individual tilt projections are collected. We have adapted a biomedical imaging approach to improve image quality by enhancing individual tilt projections prior to volumetric reconstruction. Specifically, we have developed a family of non-linear anisotropic diffusion (NAD) filters parameterized by the tilt angle. We give a quantitative and qualitative evaluation of our pre-processing approach and the NAD filter. We show an improvement in the reconstructed volumes for tomograms generated from both plastic-embedded and cryo-stabilized samples of malaria parasite-infected erythrocytes. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:132 / 142
页数:11
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