Comparative analysis of peroxidase profiles in Chinese kale (Brassica alboglabra L.): Evaluation of leaf growth related isozymes

被引：3
|
作者：
Tang, Lei [1 ]
Wang, Chenchen [1 ]
Huang, Jiabao [1 ]
Zhang, Jianhua [2 ]
Mao, Zhonggui [2 ]
Wang, Haiou [3 ]
机构：
[1] Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Sch Biotechnol, Wuxi 214122, Jiangsu, Peoples R China
[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
来源：
FOOD CHEMISTRY | 2013年 / 136卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Chinese kale; Isozymes; Peroxidase; Zymogram; CLASS-III PEROXIDASES; CHLOROPHYLL DEGRADATION; OLERACEA L; PURIFICATION; BROCCOLI; EXPRESSION; CLONING; FAMILY; GENE; LOCALIZATION;
D O I：
10.1016/j.foodchem.2012.08.054
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Plant peroxidases (EC 1.11.1.7) with different isoforms catalyze various reactions in plant growth and development. However, it is difficult to elucidate the function of each isozyme in one plant. Here, we compared profiles of entire isozyme in young seedling and mature leaves of Chinese kale (Brassica alboglabra L) on zymogram and ion exchange chromatography in order to investigate leaf growth related peroxidase isozymes. The results showed that four isozymes were constitutively expressed in kale leaves, whereas other two isozymes were induced in the mature leaves. The Mono Q ion exchange chromatography separated the six isozymes into two major groups due to the difference in their isoelectric points. The results suggested that although there were several isozymes in the leaves of Chinese kale, one isozyme functioned mainly through the leaf development. Two anionic isozymes with molecular weights lower than 32 kDa were considered mature related. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：632 / 635
页数：4
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