Surface modification by electron irradiation for improved immunoassay

被引：11
|
作者：
Safrany, A
Deelder, A
机构：
[1] Hungarian Acad Sci, Chem Res Ctr, Inst Isotope & Surface Chem, H-1525 Budapest, Hungary
[2] Leiden Univ, Med Ctr, Dept Parasitol, NL-2300 RC Leiden, Netherlands
来源：
RADIATION PHYSICS AND CHEMISTRY | 1999年 / 55卷 / 5-6期
关键词：
D O I：
10.1016/S0969-806X(99)00211-X
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Polystyrene microtitration (ELISA) plates modified by electron beam irradiation were used for a monoclonal antibody based sandwich immunoassay for quantitation of circulating anodic antigen levels in Schistosoma-infected individuals. The plates irradiated with 15 kGy showed 2-4-fold lower detection level compared to untreated plates, and a 10-fold lower antibody coating concentration than usually used was still detectable. These results were reproducible and the modified surfaces were stable even after 2 years when kept at room temperature. (C) 1999 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：681 / 684
页数：4
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